实验流体力学2026，Vol.40Issue(4)：97-104,8.DOI:10.11729/syltlx20250121
狭窄微流控通道中细胞挤压力生物学分析
Mechanobiological analysis of cellular compression in narrow microfluidic channels
摘要
Abstract
The compressive stress experienced by Circulating Tumor Cells(CTCs)in the microcirculation is a key mechanical factor affecting their metastatic potential.This study aims to investigate the independent regulatory effects of compressive stress intensity and duration on cell viability.Referring to the typical diameter of the MCF-7 cell line,microfluidic channels with different widths(9,12 and 15 µm)and lengths(100 and 500 µm)were designed to construct an in vitro mechanical stimulation platform,simulating the capillary environment and decoupling the two mechanical parameters.The dynamic process and transit time of MCF-7 cells passing through the microchannels were recorded using high-speed microimaging technology.Fluorescence staining and image analysis were employed to quantitatively evaluate the proliferation and adhesion capabilities of cells after different mechanical stimulations.Results show that the cell transit time is primarily determined by the channel length,while a decrease in channel width increases the dispersion of transit velocities.Regarding cell viability,under short-duration compressive stimulation(approx.3 ms),cell proliferation ability decreased with increasing stress intensity;whereas under long-duration stimulation(approx.15 ms),it showed a trend of first enhancing and then weakening.Furthermore,compressive stress significantly inhibited cell adhesion ability,and the effect was more pronounced with prolonged stimulation.This study provides support for deciphering the regulation of cell fate by stress stimulation intensity and time,offering experimental evidence for a deeper understanding of the metastasis mechanism of circulating tumor cells.关键词
微流控/循环肿瘤细胞/挤压应力/力学生物学/细胞活性/细胞黏附Key words
microfluidics/Circulating Tumor Cells(CTCs)/compressive stress/mechan-obiology/cell viability/cell adhesion分类
数理科学引用本文复制引用
陈亦扬,戎佳欣,龚晓波..狭窄微流控通道中细胞挤压力生物学分析[J].实验流体力学,2026,40(4):97-104,8.基金项目
国家自然科学基金重点项目(12432014) （12432014）
国家重点研发计划政府间国际科技创新合作重点专项(2025YFE0107500) （2025YFE0107500）
