林业科学研究2026，Vol.39Issue(4)：34-46,13.DOI:10.12403/j.1001-1498.20250316
毛竹BGLU家族成员鉴定及胁迫处理下的表达分析
Identification of BGLU Family Members in Moso Bamboo and Expression Analysis under Stress Treatment
摘要
Abstract
[Objective]β-glucosidase(BGLU)is a glycoside hydrolase involved in diverse plant develop-mental processes and stress responses.Elucidating the molecular characteristics and expression profiles of BGLU genes in moso bamboo(Phyllostachys edulis)is crucial for uncovering their functions in bamboo rapid growth and abiotic stress adaptation.[Methods]In this study,bioinformatics approaches were em-ployed to identify and systematically characterize the members of BGLU gene family in moso bamboo gen-ome.Transcriptome data were integrated to screen key genes,followed by construction of a co-expres-sion network.The expression patterns of key genes were analyzed using qRT-PCR technology,and the regulatory relationship was experimentally verified through yeast one-hybrid(Y1H)and dual luciferase re-porter(DLR)assays.[Results]A total of 40 members of BGLU gene family(designated as PeBGLU1 to PeBGLU40)were identified in moso bamboo genome,which distributed unevenly across 18 chromo-somes,with 15 pairs of PeBGLUs exhibiting collinearity.PeBGLUs contained 5 to 13 exons,and their en-coded proteins ranged from 274 to 979 amino acids in length,all of which were hydrophilic proteins.Phylo-genetic analysis revealed that PeBGLUs were divided into 8 groups,among which Group Ⅶ containing the largest number of members(13)and Group Ⅷ had no member of moso bamboo.The promoter se-quences of PeBGLUs harbored a variety of stress and hormone response elements.Transcriptome data analysis indicated that PeBGLUs in leaves responded to drought,salt stress,and abscisic acid(ABA)to different extents,among which the expression change of PeBGLU25 was the most significant and it was co-expressed with 7 transcription factor genes.qRT-PCR results confirmed that Pe02464_MYB and PeB-GLU25 exhibited similar expression trends.Further Y1H and DLR assays demonstrated that Pe02464_MYB could bind to the PeBGLU25 promoter and activate its expression.[Conclusion]In total,40 BGLU gene family members were identified in moso bamboo,and these genes exhibit distinct tissue-spe-cific expression patterns and abiotic stress response profiles.Notably,PeBGLU25 was transcriptionally regulated by Pe02464_MYB,suggesting that PeBGLU25 may play a critical role in the response of moso bamboo to drought,salt stress,and ABA treatment.关键词
毛竹/β-葡萄糖苷酶/分子特征/表达分析/转录调控Key words
Phyllostachys edulis/beta-glucosidase/molecular characterization/expression analysis/transcriptional regulation分类
农业科技引用本文复制引用
宛慧茹,李慧,朱成磊,寇单单,高志民..毛竹BGLU家族成员鉴定及胁迫处理下的表达分析[J].林业科学研究,2026,39(4):34-46,13.基金项目
国家重点研发计划项目课题2(2021YFD2200502)国家级 （2021YFD2200502）
