农业生物技术学报2026，Vol.34Issue(9)：1867-1876,10.DOI:10.3969/j.issn.1674-7968.2026.09.004
马铃薯干旱响应转录因子StERF89的表达及其对StDIR31的调控作用
Expression of the Drought-responsive Transcription Factor StERF89 in Potato(Solanum tuberosum)and Its Regulatory Role of StDIR31
摘要
Abstract
The AP2/ERF(APETALA2/ethylene responsive factor)transcription factor family,one of the largest transcription factor families in plants,plays a crucial role in regulating plant growth,development,and abiotic stress responses.In this study,using the potato(Solanum tuberosum)cultivar'Atlantic'as material,the S.tuberosum ethylene-responsive factor 89(StERF89)gene(GenBank No.XM_006365280)was cloned,with a CDS of 891 bp.Bioinformatics analysis revealed that this gene lacked introns,and its promoter region contained cis-acting elements associated with drought stress response.Tissue-specificity analysis showed that StERF89 expression levels were highest in potato tubers,followed by leaves.Under natural drought conditions,StERF89 gene expression exhibited an upward trend as drought stress intensified,suggesting potential involvement in drought stress response.Further analysis revealed that the promoter region of S.tuberosum dirigent protein 31(StDIR31),a lignin synthesis-related gene,contained 2 DRE/CRT elements,and its expression pattern under drought stress was similar to that of StERF89.Using yeast one-hybrid assays,dual luciferase reporter assays,and β-glucuronidase(GUS)staining experiments,it was further confirmed that StERF89 directly bound to the StDIR31 promoter and positively regulated its expression.This study provides crucial evidence for further investigation into the functional regulation of the StERF89 transcription factor.关键词
马铃薯/干旱胁迫/转录因子/马铃薯乙烯响应因子89(StERF89)/马铃薯Dirigent蛋白31(StDIR31)Key words
Potato/Drought stress/Transcription factor/Solanum tuberosum ethylene-responsive factor 89(StERF89)/Solanum tuberosum dirigent protein 31(StDIR31)分类
农业科技引用本文复制引用
王艳浩,王霄,王凯彤,魏涵,杨亮,吕硕,张宁,司怀军..马铃薯干旱响应转录因子StERF89的表达及其对StDIR31的调控作用[J].农业生物技术学报,2026,34(9):1867-1876,10.基金项目
甘肃省联合科研基金重大项目(24JRRA836) （24JRRA836）
甘肃省种业攻关项目(ZYGG-2025-7) （ZYGG-2025-7）
