农业生物技术学报2026，Vol.34Issue(9)：1877-1886,10.DOI:10.3969/j.issn.1674-7968.2026.09.005
辣椒CaBGLU21基因的克隆及表达分析
Cloning and Expression Analysis of CaBGLU21 Gene in Pepper(Capsicum annuum)
摘要
Abstract
β-glucosidase(BGLU)is a key enzyme involved in plant hormone regulation and stress response,and functional characterization of BGLU family genes is of great importance for genetic improvement of stress resistance in pepper(Capsicum annuum).Using the inbred line'17CL30'of pepper as the experimental material,this study cloned the pepper β-glucosidase 21 gene(C.annuum β-glucosidase 21,CaBGLU21)(GenBank No.PX753275.1),and performed systematic analysis on its bioinformatics characteristics,expression pattern and cis-acting elements in the promoter region.The results showed that CaBGLU21 encoded a stable alkaline hydrophilic protein containing 406 amino acids,which harbored a conserved Glyco_hydro_1 domain and multiple potential phosphorylation sites.Phylogenetic analysis indicated that CaBGLU21 had the closest genetic relationship with BGLU proteins from Solanaceae species,including Solanum lycopersicum,Nicotiana tabacum and Lycium ferocissimum.Promoter analysis revealed that the promoter sequence of CaBGLU21 contained cis-acting elements associated with light response,hormone response(MeJA,ABA,SA)and adversity stress.qRT-PCR results demonstrated that the expression of CaBGLU21 was significantly induced by various treatments including ABA,JA,IAA,GA3,H2O2,NaCl,high temperature and low temperature,with its expression level reaching the peak at around 12 h after treatment.The results of this study preliminarily showed that CaBGLU21 might play an important role in pepper's response to hormone signals and abiotic stresses.This study provides a reference for in-depth dissection of the gene's function and provided candidate gene resources for pepper breeding for stress tolerance.关键词
辣椒/基因克隆/生物信息学分析/β-葡萄糖苷酶21(BGLU21)/非生物胁迫Key words
Pepper/Gene cloning/Bioinformatics analysis/β-glucosidase 21(BGLU21)/Abiotic stress分类
农业科技引用本文复制引用
杨渊才,梁燕平,焦彦生,苗如意,田如霞..辣椒CaBGLU21基因的克隆及表达分析[J].农业生物技术学报,2026,34(9):1877-1886,10.基金项目
山西省科技创新人才团队专项计划(202304051001018) （202304051001018）
山西省重点研发计划(202402140601003) （202402140601003）
