麦类作物学报2026，Vol.46Issue(8)：991-999,9.DOI:10.7606/j.issn.1009-1041.2026.08.01
敲除TaMAPK1部分同源基因对小麦苗期耐旱性的调控机制
Regulatory Mechanism Underlying Drought Tolerance in Wheat Seedlings via TaMAPK1 Partial Homeologous Genes Knockout
摘要
Abstract
To elucidate the functions of the wheat mitogen-activated protein kinase genes TaMAPK1-6A,TaMAPK1-6B,and TaMAPK1-6Din seedling drought tolerance and the additive effects among these homeologous genes,this study utilized the spring wheat cultivar Fielder and its CRISPR/Cas9 media-ted single-gene mutants-mapk1-aa,mapk1-bb,and mapk1-dd-generated in the Fielder genetic back-ground.Through hybridization,self-pollination,and genotyping of segregating progeny,double-gene knockout mutants and triple-gene knockout homozygous mutants(hereafter referred to as double and triple mutants)were successfully developed.The drought tolerance at the seedling stage was system-atically evaluated for these single,double,and triple mutants,along with the wild-type Fielder con-trol.The results demonstrated that loss-of-function mutations in single TaMAPK1 genes significantly reduced drought tolerance in wheat seedlings,manifested as decreased leaf water retention,reduced accumulation of osmotic adjustment substances,and enhanced membrane lipid peroxidation.Further investigation revealed that drought tolerance was further impaired in double and triple mutants,with the deterioration of physiological and biochemical indicators intensifying as the number of mutated gene copies increased.In summary,the TaMAPK1 gene positively regulates drought tolerance at the wheat seedling stage,and its homeologous genes exhibit cumulative regulatory effects.关键词
小麦/TaMAPK1/耐旱性/基因编辑/同源基因/叠加效应Key words
Wheat/TaMAPK1/Drought tolerance/Gene editing/Homoeologs/Additive effect分类
农业科技引用本文复制引用
杜迪迪,吴保为,龚悦,马猛,刘香利,赵惠贤..敲除TaMAPK1部分同源基因对小麦苗期耐旱性的调控机制[J].麦类作物学报,2026,46(8):991-999,9.基金项目
陕西省重点研发项目(2025NC-YBXM-002) （2025NC-YBXM-002）
国家自然科学基金项目(32372103和32072003) （32372103和32072003）
陕西省自然科学基础研究计划项目(2024JC-YBMS-171) （2024JC-YBMS-171）
