麦类作物学报2026，Vol.46Issue(8)：1000-1013,14.DOI:10.7606/j.issn.1009-1041.2026.08.02
大麦R2R3-MYB转录因子家族鉴定及低磷胁迫下Hv2RMYB-48的功能验证
Identification of the R2R3-MYB Transcription Factor Family in Barley and Functional Verification of Hv2RMYB-48 under Low-Phosphorus Stress
摘要
Abstract
The R2R3-MYB transcription factor family,the largest subfamily of MYB transcription fac-tors,plays critical roles in various plant stress responses.However,its function under low-phosphate(P)stress remains poorly characterized.In this study,a total of 127 R2R3-MYB genes were identified in the barley MorexV3 genome using bioinformatic approaches and designated as Hv2RMYB-1 to Hv2RMYB-127.These genes were predominantly distributed on the 7 barley chromosomes and were found to harbor hormone-responsive cis-acting elements in their promoter regions.Physicochemical a-nalysis revealed that most members encode acidic and unstable proteins.Phylogenetic and collinearity analyses classified the barley R2R3-MYB family into five distinct clades,including 16 pairs of tandemly duplicated genes.Based on transcriptomic data(RNA-Seq)and quantitative real-time PCR(qRT-PCR),10 barley R2R3-MYB genes showed differential expression under low-Phosphorus stress,among which Hv2RMYB-48 was notably upregulated.Further investigation confirmed that Hv2RMYB-48 localizes to the nucleus and can bind to the MYB-ABRE-MYB cis-element.关键词
大麦/R2R3-MYB转录因子/低磷胁迫/生物信息学/酵母单杂交Key words
Barley/R2R3-MYB transcription factor/Low phosphorus stress/Bioinformatics/Yeast one-hybrid analysis分类
农业科技引用本文复制引用
王长杰,杨轲,孟亚雄,马小乐,王化俊,汪军成,汪欣瑶,陈佳佳,赵娜,王勇胜,牛丽,张宏,姚立蓉,司二静..大麦R2R3-MYB转录因子家族鉴定及低磷胁迫下Hv2RMYB-48的功能验证[J].麦类作物学报,2026,46(8):1000-1013,14.基金项目
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05-02A-02) （CARS-05-02A-02）
甘肃省现代农业产业技术体系建设项目(GSARS07) （GSARS07）
甘肃农业大学创新训练项目(202501076) （202501076）
