农业生物技术学报2026，Vol.34Issue(9)：1940-1949,10.DOI:10.3969/j.issn.1674-7968.2026.09.010
过表达BCAT1基因对奶山羊乳腺上皮细胞乳蛋白合成的影响
Effects of Overexpression of BCAT1 Gene on Milk Protein Synthesis in Mammary Epithelial Cells of Dairy Goats(Capra hircus)
摘要
Abstract
Branched-chain amino acid aminotransferase 1(BCAT1)is a key enzyme in the catabolism of branched chain amino acids(BCAAs).This study aimed to obtain the CDS sequence of the BCAT1 gene and preliminarily investigate the effect of this gene on milk protein synthesis in mammary epithelial cells of dairy goats(Capra hircus).Mammary epithelial cells of dairy goats were used as materials,the CDS region of the BCAT1 gene was cloned,and bioinformatics analysis of the CDS sequence was performed using online software.The pcDNA3.1-BCAT1 overexpression vector was constructed,and the effect of the BCAT1 gene on milk protein synthesis in mammary epithelial cells was explored.The results showed that the full-length CDS region of the BCAT1 gene was 1 161 bp,encoding 386 amino acids.The molecular weight of the BCAT1 protein was 43 173.42 D,and the theoretical pI value was 5.05.No transmembrane domain or signal peptide was detected,and it was identified as a negatively charged,hydrophobic and stable protein.Protein-protein interaction analysis showed that the BCAT1 protein interacted with branched chain keto acid dehydrogenase E1 subunit alpha(BCKDHA),BCKDHB and other proteins.Tissue expression profile analysis indicated that the expression level of the BCAT1 gene was relatively high in mammary gland tissue during the dry period.After transfection of the BCAT1 gene recombinant overexpression vector into mammary epithelial cells,the expression levels of milk protein-related genes,including beta-lactoglobulin(BLG)(P<0.01),casein alpha s1(CSN1S1)(P<0.05),casein beta(CSN2)(P<0.05),and casein kappa(CSN3)(P<0.05),were significantly increased.It was indicated that the BCAT1 gene played a regulatory role in milk protein synthesis in goat mammary epithelial cells.This study provides a theoretical foundation for further research on the regulatory mechanism of the BCAT1gene in goat milk protein synthesis.关键词
分支链氨基酸转氨酶1(BCAT1)/乳蛋白合成/山羊乳腺上皮细胞/奶山羊Key words
Branched-chain-amino-acid aminotransferase 1(BCAT1)/Milk protein synthesis/Goat mammary epithelial cells(GMECs)/Dairy goats(Capra hircus)分类
农业科技引用本文复制引用
宋伊敏,史怀平,刘铮铸,张会文,李宏强,王东贤,刘泗江,郭子龙,罗妤,李肖依颜,邵钺馨,刘艳新..过表达BCAT1基因对奶山羊乳腺上皮细胞乳蛋白合成的影响[J].农业生物技术学报,2026,34(9):1940-1949,10.基金项目
河北省现代农业产业技术体系建设专项 （）
河北科技师范学院科学研究基金(2025YB012) （2025YB012）
河北省自然科学青年基金(C2025407018) （C2025407018）
