农业生物技术学报2026，Vol.34Issue(9)：1950-1960,11.DOI:10.3969/j.issn.1674-7968.2026.09.011
响应面优化牛骨肽提取工艺对疲劳训练比格犬生殖损伤的研究
Response Surface Optimization of Bovine Bone Peptide Extraction Process for Studying Reproductive Damage in Fatigued Beagle Dogs(Canis lupus familiaris)
摘要
Abstract
As one of the high-workload functional dogs,police dogs(Canis lupus familiaris)typically perform demanding tasks during routine training and missions,often accompanied by issues such as decreased endurance and reproductive disorders.Therefore,this study employed response surface methodology to optimize the extraction of bovine bone peptide(BBP),subsequently investigated its effects on preventing fatigue-induced reproductive damage in fatigued Beagle dogs.Beagles were randomly divided into 4 groups:Normal(normal feeding,no training),Model(exhaustion training),BBP-L(exhaustion training+5 g/kg BBP),and BBP-H(exhaustion training+10 g/kg BBP).Beagles underwent 4 weeks of BBP supplementation combined with downhill treadmill training at a 20° incline and 30 m/min speed,for 30 minutes daily,5 days per week.After 4 weeks,a 30-minute non-weight-bearing exhaustive swimming test was conducted.At the conclusion of the experiment,blood samples were collected from the dogs,and testes were removed to assess sperm quality within the epididymides.Serum levels of blood glucose,blood urea nitrogen(BUN),lactic acid(LA),and lactate dehydrogenase(LDH)were measured in the Beagles.Measure pyruvate kinase(PK),succinate dehydrogenase(SDH),malondialdehyde(MDA),and superoxide dismutase(SOD)levels in testicular homogenates;Detect glycogen content in liver and muscle tissues;Finally,perform hematoxylin and eosin(HE)staining and immunohistochemical staining on testicular tissues from experimental dogs to analyze the effects of BBP on testicular histology and zonula occludens-1(ZO-1)expression.The highest extraction rate of BBP was achieved under the following conditions:Boiling for 5 h,pH 10 during enzymatic hydrolysis,hydrolysis temperature of 55℃,and hydrolysis time of 6 h,yielding a rate of(35.12±1.25)％.Sperm quality and oxidative stress indicators revealed that after exhaustion training,the total sperm count,sperm density,sperm motility,and SOD levels in the trained dogs were significantly reduced(P<0.01),while MDA levels were extremely significantly elevated(P<0.001).These indicators were reversed after BBP supplementation.Serum biochemical indicators revealed that after exhaustive training,blood glucose,liver glycogen,muscle glycogen,SDH,and PK levels significantly decreased(P<0.01),while BUN,LA,and LDH levels significantly increased(P<0.01).After supplementation with BBP,blood glucose,liver glycogen,muscle glycogen,SDH,and PK levels significantly increased(P<0.01),while BUN,LA,and LDH levels significantly decreased(P<0.01).Histopathological examination revealed enlarged intercellular spaces and loose tissue structure in testicular supporting cells,accompanied by seminiferous tubule abnormalities.ZO-1 expression was significantly downregulated(P<0.01),with marked improvement after BBP supplementation(P<0.01).This study possesses important application value for alleviating problems such as endurance decline and reproductive dysfunction in police dogs during duty operations and high-intensity training.关键词
牛骨肽(BBP)/响应面优化/抗犬疲劳/生殖损伤Key words
Bovine bone peptide(BBP)/Response surface optimization/Anti-canine fatigue/Reproductive injury分类
农业科技引用本文复制引用
谢留威,李宝安,宋明强,范冰峰,邢宝瑞,刘庆,孙宁,武振龙..响应面优化牛骨肽提取工艺对疲劳训练比格犬生殖损伤的研究[J].农业生物技术学报,2026,34(9):1950-1960,11.基金项目
中央高校基本科研业务费(D2026068 （）
D2024005) （）
公安部重点研发计划(2024JSZ12) （2024JSZ12）
十四五国家重点研发计划(2024YFC3307000) （2024YFC3307000）
