农业生物技术学报2026，Vol.34Issue(9)：2005-2017,13.DOI:10.3969/j.issn.1674-7968.2026.09.015
紧密连接蛋白Occludin促进猪德尔塔冠状病毒感染ST细胞的机制
Mechanisms Underlying the Promotion of Porcine deltacoronavirus Infection in ST Cells by Tight Junction Protein Occludin
摘要
Abstract
Porcine deltacoronavirus(PDCoV)is an emerging enteric coronavirus that causes acute watery diarrhea,vomiting,and dehydration in neonatal piglets(Sus scrofa).PDCoV infection leads to villus atrophy,crypt hyperplasia,and disruption of the intestinal barrier,resulting in high mortality and substantial economic losses to the swine industry.Occludin,a key component of tight junctions,plays critical roles in maintaining epithelial barrier integrity and mediating signal transduction.Using swine testicular(ST)cells as a model,the effect of PDCoV infection on Occludin expression was assessed by Western blot and qRT‑PCR analyses in this study.Results demonstrated that both Occludin mRNA and protein levels decreased significantly over time post‑infection(P<0.01),suggesting a correlation between PDCoV infection and Occludin downregulation.To further clarify the functional role of Occludin in viral replication,Occludin overexpressed or knocked down via plasmid overexpression and short hairpin RNA(shRNA)interference,respectively.The results showed that Occludin overexpression significantly promoted PDCoV infection(P<0.0001),while Occludin knockdown significantly inhibited PDCoV infection(P<0.0001),indicating that Occludin was a critical host factor involved in PDCoV replication.Furthermore,to explore the entry pathways through which Occludin promotes viral infection,cells were treated with the clathrin inhibitor Pitstop 2 and the macropinocytosis inhibitor 5‑(N‑ethyl‑N‑isopropyl)amiloride(EIPA).Both inhibitors markedly attenuated the Occludin overexpression‑mediated enhancement of PDCoV infection.Collectively,The findings elucidated that Occludin positively regulated PDCoV entry via clathrin‑mediated endocytosis and macropinocytosis,providing a theoretical basis for in‑depth understanding of PDCoV pathogenesis and for developing novel host factor‑targeted control strategies.关键词
猪德尔塔冠状病毒(PDCoV)/Occludin/内吞/紧密连接/致病机制Key words
Porcine deltacoronavirus(PDCoV)/Occludin/Endocytosis/Tight junction/Pathogenic mechanism分类
农业科技引用本文复制引用
张雪莉,夏良星,张帅,包文斌..紧密连接蛋白Occludin促进猪德尔塔冠状病毒感染ST细胞的机制[J].农业生物技术学报,2026,34(9):2005-2017,13.基金项目
国家自然科学基金青年科学基金(32402729) （32402729）
