农业生物技术学报2026，Vol.34Issue(9)：2030-2039,10.DOI:10.3969/j.issn.1674-7968.2026.09.017
三种不同载体介导蝴蝶兰瞬时转化效果的比较研究
Comparative Study on the Efficiency of Transient Transformation in Phalaenopsis spp.Mediated by Three Distinct Vector
摘要
Abstract
Phalaenopsis spp.suffers from complex genetic backgrounds and difficult in vitro regeneration,compounded by the low efficiency of traditional stable genetic transformation,which hampers research on gene function and molecular breeding.Virus vector-mediated transient transformation technology offers an effective approach to address these issues.In this study,Phalaenopsis'Zishuijing'was used to compare the transient genetic transformation efficiencies mediated by 3 viral vectors:Cymbidium mosaic virus(CymMV),Tobacco rattle virus(TRV),and Cabbage leaf curl virus(CaLCuV).Recombinant vectors carrying the phytoene desaturase(PDS)gene were introduced into leaves via Agrobacterium-mediated injection.The silencing efficiency of the 3 vectors was evaluated based on the photobleaching phenotype induced by PDS silencing and analysis of gene expression levels.The results indicated that significant differences in performance among the 3 vectors,with pCymMV demonstrating the highest transformation efficiency.Color fading was observed on inoculated leaves 4 weeks post-inoculation,and albinism emerged in newly developed leaves at 7 weeks.Moreover,the expression level of PePDS showed a nearly linear decreasing trend.In conclusion,pCymMV was the optimal vector for mediating transient transformation in Phalaenopsis.This study provides an efficient technical foundation for gene function analysis and molecular breeding in Phalaenopsis.关键词
蝴蝶兰/瞬时转化/建兰花叶病毒(CymMV)/烟草脆裂病毒(TRV)/甘蓝卷叶病毒(CaLCuV)/八氢番茄红素脱氢酶(PDS)基因Key words
Phalaenopsis orchid/Instantaneous conversion/Cymbidium mosaic virus(CymMV)/Tobacco rattle virus(TRV)/Cabbage leaf curl virus(CaLCuV)/Phytoene desaturase(PDS)gene分类
农业科技引用本文复制引用
毛盈,施秋阳,崔永一,辛静静..三种不同载体介导蝴蝶兰瞬时转化效果的比较研究[J].农业生物技术学报,2026,34(9):2030-2039,10.基金项目
国家自然科学基金青年科学基金(32201610) （32201610）
湖州市农业农村局重点研发项目(2025XZZD02) （2025XZZD02）
浙江省"尖兵""领雁"研发攻关计划(2023C02028) （2023C02028）
