上海针灸杂志2026，Vol.45Issue(8)：901-910,10.DOI:10.13460/j.issn.1005-0957.2026.13.5001
隔药灸对溃疡性结肠炎大鼠中性粒细胞胞外诱捕网的调节作用研究
Study on the regulatory effect of herb-partitioned moxibustion on neutrophil extracellular traps in ulcerative colitis rats
摘要
Abstract
Objective To investigate the immunomodulatory mechanism of herb-partitioned moxibustion in inhibiting inflammation in ulcerative colitis(UC)from the perspective of neutrophil extracellular trap(NET)formation.Method Male Sprague-Dawley(SD)rats were selected and randomized into a normal group(NG),a model group(MG),an herb-partitioned moxibustion group(HPM),and a medicine group(MED),with 8 rats per group.The UC model was induced using Dextran sulfate sodium(DSS).After successful model establishment,the HPM received herb-partitioned moxibustion,and the MED was intervened with Sulfasalazine enteric-coated tablets via gavage.Rats in each group were observed for their general condition,gross morphological damage of the colon,disease activity index(DAI),and colonic histopathology.Enzyme-linked immunosorbent assay(ELISA)was adopted to detect the concentrations of NET-associated complexes[cell-free DNA(cf-DNA)and myeloperoxidase(MPO)-DNA]in peripheral blood neutrophil culture supernatants and plasma,as well as the protein concentrations of pro-inflammatory cytokines[interleukin-6(IL-6)and interferon-gamma(IFN-γ)]and anti-inflammatory cytokines[interleukin-4(IL-4)]in plasma.Immunofluorescence was used to determine the co-expression of the NET's characteristic marker proteins[MPO,neutrophil elastase(NE),and citrullinated histone H3(CitH3)]in the colonic tissues of each group.Result Compared to the NG,the MG presented incomplete colonic epithelium,notable ulcer formation,significant inflammatory cell infiltration,and significantly increased DAI,colonic gross morphological damage scores,and plasma IL-6 and IFN-γ concentrations(P＜0.05),as well as markedly decreased plasma IL-4 concentrations(P＜0.05);in the MG,the concentrations of cf-DNA and MPO-DNA complexes in peripheral blood neutrophil culture supernatant and plasma were significantly elevated(P＜0.05),and the expression levels of MPO,CitH3,and NE proteins were increased at colonic ulcers infiltrated by massive neutrophils(P＜0.05),presenting obvious co-expression.Compared to the MG,the HPM and MED showed colonic mucosa covered by regenerated epithelium,healed ulcers,and decreased DAI,colonic gross morphological damage scores,and plasma IL-6 and IFN-γ concentrations(P＜0.05),as well as notably increased plasma IL-4 concentrations(P＜0.05);the two intervention groups also exhibited significant decreases in the concentrations of cf-DNA and MPO-DNA complexes in peripheral blood neutrophil culture supernatant and plasma(P＜0.05),along with reduced expression levels of MPO,CitH3,and NE proteins at colonic ulcers infiltrated by neutrophils(P＜0.05),with only a small amount of co-expression.Conclusion Herb-partitioned moxibustion can regulate the expression of colonic MPO,CitH3,and NE proteins,down-regulate the concentrations of cf-DNA and MPO-DNA complexes in peripheral blood,and inhibit excessive clustering of NETs in colonic tissues,which may be one of the main mechanisms of herb-partitioned moxibustion alleviating colonic inflammation in UC rats.关键词
灸法/隔药灸/结肠炎,溃疡性/中性粒细胞/中性粒细胞胞外诱捕网/免疫调节Key words
Moxibustion/Herb-partitioned moxibustion/Colitis,Ulcerative/Neutrophils/Neutrophil extracellular trap/Immunomodulation分类
医药卫生引用本文复制引用
马喆,钱晴兰,邓指辉,吴璐一,吴焕淦,潘卉,黄艳,钟蕊,张玥,冒金锋,祁琴,陈嫣霞..隔药灸对溃疡性结肠炎大鼠中性粒细胞胞外诱捕网的调节作用研究[J].上海针灸杂志,2026,45(8):901-910,10.基金项目
国家自然科学基金面上项目(82174501) （82174501）
国家自然科学基金优秀青年项目(82422074) （82422074）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023068) （zyyzdxk-2023068）
