生物技术通报2026，Vol.42Issue(8)：133-141,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1113
糜子伪响应调节蛋白PRR基因家族的鉴定及对光周期的响应
Identification of the Pseudo-response Regulator PRR Gene Family in Broomcorn Millet and Its Response to Photoperiod
摘要
Abstract
[Objective]Pseudo-response regulators(PRRs)play crucial roles in regulating circadian rhythms and flowering time in plants.This study aimed to systematically identify the PRR gene family in broomcorn millet(Panicum miliaceum L.)at the whole-genome level,providing a theoretical basis for understanding their biological functions.[Method]Bioinformatics methods were employed to analyze the physicochemical properties,conserved motifs,gene structures,collinearity,and phylogenetic relationships of the PRR gene family in broomcorn millet.The expression patterns of PRR genes under different photoperiod treatments were investigated using real-time quantitative PCR(RT-qPCR).[Result]A total of seven PRR genes were identified from the broomcorn millet genome,designated as PmPRR1 to PmPRR7,which were unevenly distributed across five chromosomes.Phylogenetic analysis revealed that the PmPRR genes belong to Group 1 to Group 3 subfamilies.Analysis of conserved protein motifs showed that six genes contained Motif1,Motif2,and Motif3,indicating high conservation among them.Promoter cis-acting element analysis suggested that PmPRR genes are associated with light response,plant growth and development,stress responses,and hormone regulation.RT-qPCR results demonstrated significant differences in the expressions of PmPRR genes under different photoperiod treatments(short-day,8 h;intermediate-day,14 h;long-day,16 h).[Conclusion]This study identified seven PmPRR genes.The expression patterns of genes from different subfamilies vary under different photoperiods,indicating that PmPRRs play an important regulatory role in the response of broomcorn millet to photoperiod changes.关键词
糜子/伪响应调节蛋白/光周期/生物信息学/实时荧光定量PCRKey words
broomcorn millet/pseudo-response regulators/photoperiod/bioinformatics/RT-qPCR引用本文复制引用
崔秀妍,代春阳,张谊婷,田翔,陈凌,乔治军,王海岗,王君杰..糜子伪响应调节蛋白PRR基因家族的鉴定及对光周期的响应[J].生物技术通报,2026,42(8):133-141,9.基金项目
中央引导地方科技发展资金项目(YDZJSX20231A035),山西农业大学"科技创新提升工程"项目(CXGC2023095) （YDZJSX20231A035）
