生物技术通报2026，Vol.42Issue(8)：207-216,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1168
NtPLA2A介导激素信号通路增强烟草青枯病抗性的机制
Mechanism of NtPLA2A-mediated Hormone Signaling Pathway in Enhancing Tobacco Resistance to Bacterial Wilt
摘要
Abstract
[Objective]To clone the NtPLA2A gene from tobacco(Nicotiana tabacum)and explore the molecular mechanism by which it mediates hormone signaling pathways to enhance tobacco resistance to bacterial wilt.[Method]This study cloned the NtPLA2A gene from tobacco,conducted bioinformatic analysis and expression profiling under Ralstonia solanacearum infection,and investigated the role of NtPLA2A in disease resistance using virus-induced gene silencing(VIGS)in N.benthamiana.[Result]The NtPLA2A coding sequence is 1 284 bp,encoding 428 amino acids.The protein has a molecular weight of 46.57 kD and a theoretical isoelectric point of 8.74,and is a stable hydrophilic protein.Bioinformatic analysis revealed that NtPLA2A contains a typical Patatin-like phospholipase domain and shares high amino acid sequence similarity with PLA proteins from other species.Promoter analysis indicated that the promoter region of NtPLA2A contains environmental factors such as light,low temperature,and hormones.Following R.solanacearum infection,NtPLA2A was significantly upregulated in both resistant and susceptible tobacco materials to varying degrees.Additionally,treatments with salicylic acid,jasmonic acid,and ethylene also induced its expression.VIGS results demonstrated that silencing NtPLA2A compromised resistance to R.solanacearum in N.benthamiana.[Conclusion]This study clarified the sequence,structure,and expression characteristics of tobacco NtPLA2A gene,which is induced by R.solanacearum and SA/JA/ET;its silencing significantly impairs N.benthamiana resistance to bacterial wilt.关键词
烟草/NtPLA2A基因/青枯菌/基因克隆/病毒诱导的基因沉默Key words
tobacco/NtPLA2A gene/Ralstonia solanacearum/gene cloning/virus-induced gene silencing(VIGS)引用本文复制引用
张仲文,杨爱国,王卫民,肖志亮,史久长,郝贤伟,王威,谢鹏岗,许佳丹,赵炯平..NtPLA2A介导激素信号通路增强烟草青枯病抗性的机制[J].生物技术通报,2026,42(8):207-216,10.基金项目
中国烟草总公司重大专项项目(110202201008(JY-08)),浙江中烟工业有限责任公司科技项目(ZJZY2023B004),中国烟草总公司山东省公司科技重点项目(KN304-202303),中国农业科学院科技创新工程(ASTIP-TRIC01) （110202201008(JY-08）
