实验动物与比较医学2026，Vol.46Issue(4)：507-514,8.DOI:10.12300/j.issn.1674-5817.2025.179
寒冷环境对胃肠爆震复合伤模型比格犬的病理生理影响
Pathophysiological Effects of Cold Environment on Gastrointestinal Blast Combined Injury in Beagle Dogs
摘要
Abstract
Objective To investigate the impact of a cold environment on the pathophysiological changes in dogs after abdominal gastrointestinal blast injury.Methods Eight healthy common-grade adult male Beagles were selected and randomly divided into a cold group and a normal temperature group,with 4 dogs in each group.Prior to surgery,Beagles dogs were fasted and deprived of water for 12 hours;after establishing a vein at the edge of the ear,propofol(2 mg·kg-1·min-1)was continuously infused using a microinfusion pump to maintain anesthesia.Tracheal intubation was performed while preserving spontaneous breathing,and the electroencephalogram bifrequency index was maintained at 40-60 to ensure stable anesthesia.A model of abdominal gastrointestinal blast injury in Beagle dogs was established using a multifunctional combat trauma modeling platform.After blast injury,the cold group was exposed to a cold chamber at-20℃combined with 10%body surface area ice water immersion for 40 min,while the normal temperature group was kept in an environment at(25±2)℃for 40 min after blast injury.Vital signs such as heart rate,blood pressure,and blood oxygen saturation were monitored in both groups before blast injury,immediately after blast injury,and 40 min after blast injury.And arterial blood gas parameters,including pH,partial pressure of oxygen in arterial blood(PaO2),partial pressure of carbon dioxide in arterial blood(PaCO2),actual bicarbonate radical(HCO3-),lactic acid(Lac),and blood base excess(BEb),were measured.After the experiment,gastrointestinal tissues(gastric antrum and upper jejunum 5 cm distal to the suspensory ligament of the duodenum)were harvested for pathological analysis via hematoxylin-eosin(HE)staining combined with semi-quantitative scoring.Results Physiological indicators showed that the body temperature of the cold group was significantly lower than that of the normal temperature group(P<0.01),and the heart rate and systolic blood pressure of the cold group were significantly higher than those of the normal temperature group(P<0.000 1).PaCO2 was markedly elevated and BEb was markedly decreased in the cold group relative to the normal temperature group(P<0.000 1),suggesting aggravated respiratory acidosis and metabolic acidosis.Pathological analysis showed that gastro-intestinal tissue damage was more severe in the cold group:the gastric mucosal epithelial shedding rate was>60%(vs.<25%in the normal temperature group),the necrosis rate of the epithelial cells at the tips of intestinal villi reached more than 70%(vs.<30%in the normal temperature group),and inflammatory cell infiltration involved the submucosa(vs.limited to the superficial mucosa in the normal temperature group).The semi-quantitative scoring results were consistent with the pathological analysis results,further confirming that the cold environment exacerbated gastrointestinal tissue damage caused by abdominal gastrointestinal blast injury.Conclusion A cold environment can exacerbate circulatory dysfunction and gastrointestinal tissue damage in Beagle dogs after blast injury,providing experimental evidence for early rewarming intervention for combat trauma in cold regions.关键词
寒冷环境/胃肠组织/爆震伤/复合伤/比格犬Key words
Cold environment/Gastrointestinal tissue/Blast injury/Combined injury/Beagle dogs分类
医药卫生引用本文复制引用
宫田田,王馨培,郭天革,安洋,孟子皓,姚明辉,张贺..寒冷环境对胃肠爆震复合伤模型比格犬的病理生理影响[J].实验动物与比较医学,2026,46(4):507-514,8.基金项目
北部战区总医院自主科研项目 （）
