生物技术通报2026，Vol.42Issue(8)：312-322,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1367
一株耐盐碱卡氏芽胞杆菌的分离及其促生功能鉴定
Isolation and Plant Growth-promoting Characterization of a Salt-alkali Tolerant Bacillus cabrialesii Strain
摘要
Abstract
[Objective]This study aimed to isolate and screen salt-alkali tolerant plant growth-promoting rhizobacteria,thereby providing microbial resources and theoretical foundations for the development of functional microbial fertilizers and the remediation of saline-alkali soils.[Method]Rhizosphere soil samples were collected from cotton plants in Yingjisha County,Xinjiang.Salt-alkali tolerant bacteria were isolated and screened using the serial dilution method.The strains were identified by 16S rRNA gene sequencing,and their taxonomic status was determined based on morphological,physiological,and biochemical characteristics.Salt-alkali tolerance of the strain was assessed using media with NaCl concentrations of 0‒200 g/L and pH values of 4.0‒13.0.Plant growth-promoting traits,such as phosphate solubilization,siderophore production,nitrogen fixation,biofilm formation,and amylase and cellulase activities,were evaluated using specific functional media.Finally,the effect of the strain on tomato seedling growth was examined through plate and pot experiments.[Result]We isolated a salt-alkali tolerant strain.16S rRNA gene sequence analysis showed that the strain shared 99.72%similarity with the type strain Bacillus cabrialesii TE3T,and we identified it as B.cabrialesii zp-4245.Strain zp-4245 could grow under conditions of 80 g/L NaCl and pH 9.0,and exhibited multiple plant growth-promoting traits,including siderophore production,nitrogen fixation,and the production of amylase,cellulase,and biofilm.In plate assays,we found that strain zp-4245 significantly promoted the growth of tomato seedlings under different salt-alkali stress conditions.Compared with the control at pH 5.8,inoculation with the strain increased lateral root number,aboveground fresh weight,underground fresh weight,and total fresh weight by 284.3%,87.3%,118.4%,and 94.2%,respectively.At pH 8.0,the increases were 361.7%,92.3%,141.7%,and 101.9%,respectively.Under combined stress of pH 8.0 and 2.925 g/L NaCl,the lateral root number increased by 707.6%compared to the control.Pot experiments demonstrated the growth-promoting effects of strain zp-4245 on tomato plants.Under non-salt-stress conditions,inoculation with strain zp-4245 increased aboveground fresh weight and plant height by 11.7%and 13.9%,respectively.Under salt stress(6 g/kg NaCl),inoculation increased underground fresh weight and plant height by 28.6%and 10.7%,respectively,compared with the control.[Conclusion]B.cabrialesii zp-4245 exhibits excellent salt-alkali tolerance and plant growth-promoting capabilities,significantly enhancing tomato seedling growth under varied saline-alkaline conditions.These findings provide valuable microbial resources and a theoretical foundation for developing microbial fertilizers for saline-alkali soils improvement.关键词
植物根际促生细菌/盐碱地/Bacillus cabrialesii/番茄Key words
plant growth-promoting rhizobacteria/saline-alkali soil/Bacillus cabrialesii/tomato引用本文复制引用
何浩迪,梁振普,张国只,房世杰,张小霞..一株耐盐碱卡氏芽胞杆菌的分离及其促生功能鉴定[J].生物技术通报,2026,42(8):312-322,11.基金项目
新疆维吾尔自治区重点研发专项(2022B02019) （2022B02019）
