天然产物研究与开发2026，Vol.38Issue(8)：1629-1639,11.DOI:10.16333/j.1001-6880.2026.8.001
睡莲总多酚对脂多糖诱导的RAW 264.7细胞炎症的保护机制
Preventive effect of the total polyphenols from Nymphaea candida in lipopolysaccharide-induced inflammation in RAW 264.7 cells
摘要
Abstract
This study aims to investigate the anti-inflammatory mechanism of total polyphenols from Nymphaea candida(NCTP)by using lipopolysaccharide(LPS)-induced RAW 264.7 cell inflammation model.In vitro,CCK-8 assay was used to test cell viability;the Griess reagent method was used to detect the content of nitric oxide(NO)in cell supernatant;ELISA methods were used to measure the levels of cyclooxygenase-2(COX-2),prostaglandin E2(PGE2),interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in the cell supernatant;immunofluorescence and flow cytometry were used to measure mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)levels;flow cytometry was used to test M1 polarization;Western blot was utilized to determine the protein expression levels of Toll-like receptor 4(TLR4),mye-loid differentiation factor 88(MyD88),transforming growth factor β-activated kinase 1(TAK1),inhibitor of κB kinase(IKK),inhibitor of NF-κB(IκB),nuclear factor-κB(NF-κB),extracellular regulated protein kinases(ERK),c-Jun N-ter-minal kinase(JNK),P38 mitogen-activated protein kinase(P38 MAPK),NOD-like receptor protein 3(NLRP3),apoptosis-associated speck-like protein containing a CARD(ASC),cysteinyl aspartate specific proteinase-1(Caspase-1),heme oxyge-nase-1(HO-1),Kelch-like ECH-associated protein 1(KEAP1)and nuclear factor erythroid 2-related factor 2(NRF2).The results showed that NCTP could inhibit the levels of NO,PGE2,COX-2,IL-1β,IL-6 and TNF-α(P＜0.01);and prevented LPS-induced RAW 264.7 macrophages M1 polarization(P＜0.01).NCTP also downregulated the expression of TLR4,MyD88,TAK1,IKK,p-IκB/IκB,p-NF-κB/NF-κB,p-JNK/JNK,p-P38 MAPK/P38 MAPK,NLRP3,ASC,Caspase-1,KEAP1 proteins,and upregulated the expression of NRF2 and HO-1 in RAW 264.7 cell(P＜0.05,P＜0.01).Meanwhile,NCTP el-evated MMP and reduced ROS production(P＜0.05,P＜0.01).These results demonstrated that anti-inflammatory mecha-nism of NCTP might be closely related to antioxidation,ameliorating mitochondrial function,and regulating TLR4/MAPK/NF-κB,NLRP3,KEAP1/NRF2/HO-1 pathways.关键词
睡莲花/睡莲总多酚/炎症/TLR4/MAPK/NF-κB/NLRP3/RAW 264.7细胞Key words
Nymphaea candida Presl/total polyphenols from Nymphaea candida/inflammation/TLR4/MAPK/NF-κB/NL-RP3/RAW 264.7 cells引用本文复制引用
李晨阳,姚雨含,艾迪达尔·托力肯,陈燕,赵军..睡莲总多酚对脂多糖诱导的RAW 264.7细胞炎症的保护机制[J].天然产物研究与开发,2026,38(8):1629-1639,11.基金项目
新疆维吾尔自治区自然科学基金重点项目(2021D01D14) （2021D01D14）
新疆维吾尔自治区公益性科研院所基本科研业务费项目(KY2025083) （KY2025083）
