天然产物研究与开发2026，Vol.38Issue(8)：1679-1686,1705,9.DOI:10.16333/j.1001-6880.2026.8.006
二氢杨梅素抑制斑马鱼尾静脉血栓形成的作用与机制
Inhibitory effects and mechanisms of dihydromyricetin against caudal venous thrombosis in zebrafish
摘要
Abstract
This study aims to elucidate the effect and mechanism of dihydromyricetin(DHM)against venous thrombosis in zebrafish.Zebrafish at 3 days post-fertilization were randomly divided into six groups:a blank control,model,DHM low,medi-um,and high dose groups(50,100,and 200 μg/mL),and an aspirin group(25 μg/mL).After six hours of treatment with DHM or aspirin,all groups except the blank control were exposed to 30 μg/mL arachidonic acid for 1 hour to induce caudal vein thrombosis.The caudal vein and cardiac erythrocytes of zebrafish were stained to evaluate the antivenous thrombosis effect of dihydromyricetin(DHM),live fluorescence imaging was employed to observe neutrophil activation and recruitment,RT-qPCR was performed to determine the mRNA expression levels of myeloperoxidase(MPO),tumor necrosis factor-alpha(TNF-α),and interleukin-6(IL-6),reactive oxygen species(ROS)levels were measured using a fluorescent probe assay,intestinal hemorrhage was examined under a microscope to assess the safety of DHM,molecular docking was conducted to ver-ify its binding activity with key targets.Results showed that compared to the model group,DHM significantly reduced thrombus formation in the caudal vein(P＜0.01),increased cardiac red blood cell staining area(P＜0.01),decreased neu-trophil fluorescence intensity(P＜0.01),and suppressed mRNA expression of MPO(P＜0.001),TNF-α(P＜0.001),and IL-6(P＜0.01).DHM also markedly inhibited ROS generation(P＜0.001).DHM did not cause intestinal bleeding at any tested concentrations.Molecular docking indicated that DHM exhibited favorable binding affinities with MPO,TNF-α,IL-6,and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2).These findings suggest that DHM may reduce caudal vein thrombosis in zebrafish by inhibiting the TNF-α/IL-6-ROS-MPO signaling axis,without inducing hemorrhagic effects.关键词
静脉血栓栓塞症/斑马鱼/二氢杨梅素/中性粒细胞胞外诱捕网/髓过氧化物酶/炎症反应Key words
venous thromboembolism/Zebrafish/Dihydromyricetin/neutrophil extracellular traps/myeloperoxidase/inflammation分类
医药卫生引用本文复制引用
资石祥,陈彩艳,李文,陈云志,陈帅..二氢杨梅素抑制斑马鱼尾静脉血栓形成的作用与机制[J].天然产物研究与开发,2026,38(8):1679-1686,1705,9.基金项目
国家自然科学基金(82360860) （82360860）
贵州省自然科学基金(黔科合基础-ZK[2023]一般405) （黔科合基础-ZK[2023]一般405）
贵州省卫生健康委科学技术基金(gzwkj2022-481) （gzwkj2022-481）
国家级大学生创新创业训练计划(202310662135) （202310662135）
