天然产物研究与开发2026，Vol.38Issue(8)：1777-1789,13.DOI:10.16333/j.1001-6880.2026.8.016
基于网络药理学和非靶向代谢组学探讨白术-苍术改善ob/ob小鼠肥胖的作用机制
Mechanism of Atractylodis Macrocephalae Rhizoma-Atractylodis Rhizoma in improving obesity of ob/ob mice based on network pharmacology and untargeted metabolomics
摘要
Abstract
Based on network pharmacology and untargeted metabolomics,the mechanism of Atractylodis Macrocephalae Rhizo-ma-Atractylodis Rhizoma(AM-AL)herb pair improving obesity was explored.The ob/ob mice model of obesity was used.Micro CT was employed to analyze the volumes of inguinal white adipose tissue(iWAT)and epididymal white adipose tissue(eWAT).Masson staining was used to observe the pathological changes of eWAT.ELISA was performed to detect the levels of interleukin-6(IL-6),IL-17,tumor necrosis factor-α(TNF-α),and malondialdehyde(MDA)in eWAT.Q-Orbitrap high-resolution liquid chromatography-mass spectrometry was used to qualitatively analyze the absorbed components of AM-AL in serum.Network pharmacology technology was used to screene core targets between absorbed components and diseases.Untar-geted metabolomics was used to screene differential metabolites of eWAT,and related metabolic pathways.Combined with net-work pharmacology,a"metabolite-reaction-enzyme-gene"network was constructed for joint analysis.The animal experiment results showed that the high-dose AM-AL group could reduce the body weight and daily food intake of mice;reduce the vol-ume of iWAT and eWAT;and improve the levels of inflammation and oxidative stress.Q-Orbitrap identified 49 compounds in serum.Network pharmacology results showed that AKT serine/threonine kinase 1(AKT1)was the core target,mainly invol-ving metabolic pathways,lipids and atherosclerosis,and so on.Untargeted metabolomics showed that AM-AL significantly reg-ulated 20 differential metabolites.The integrated analysis results indicated that glycerophospholipid metabolism might be the key pathway for the anti-obesity effect of AM-AL.This experiment initially clarified the mechanism of AM-AL in improving o-besity,providing a scientific basis for its clinical application and subsequent research.关键词
白术-苍术/肥胖/Q-Orbitrap高分辨液质联用/网络药理学/非靶向代谢组学Key words
Atractylodis Macrocephalae Rhizoma-Atractylodis Rhizoma/obesity/Q-Orbitrap high-resolution liquid chromatog-raphy-mass spectrometry/network pharmacology/untargeted metabolomics分类
医药卫生引用本文复制引用
李静,许宗颖,徐进文,骆欢欢..基于网络药理学和非靶向代谢组学探讨白术-苍术改善ob/ob小鼠肥胖的作用机制[J].天然产物研究与开发,2026,38(8):1777-1789,13.基金项目
国家自然科学基金(82174244,81774107) （82174244,81774107）
院校共建重点项目(GZYZS2024U03) （GZYZS2024U03）
