湖北科技学院学报（医学版）2026，Vol.40Issue(4)：316-320,331,6.DOI:10.16751/j.cnki.2095-4646.2026030406
高脂饮食诱导肥胖小鼠模型中短链脂肪酸的动态观察
Dynamic Changes of Short-Chain Fatty Acids in Obese Mouse Model Induced by High-Fat Diet
摘要
Abstract
Objective To observe the dynamic changes of short-chain fatty acids(SCFAs)in obese mice induced by high-fat diet,and and to analyze these changes in conjunction with gut microbiota,in order to explore the correlation between SCFAs and obesity.Methods Sixteen male C57BL/6 mice were randomly divided into control group and model group,with8 mice in each group.The model group was fed with high-fat diet to construct obesity model;the control group was fed with normal diet for 10 weeks.The body weight of mice was weighed regularly every week,and fresh feces were collected.The content of SCFAs in feces was dynamically detected by liquid chromatography-mass spectrometry.At the end of the experiment,the serum biochemical indexes of TG,TC,LDL-C and HDL-C were measured;The pathological characteristics of epididymal white adipose tissue were observed by HE staining.The composition and diversity of microbial communities in the cecum of mice were detected by 16S rRNA high-throughput sequencing.Results The body weight of mice in the model group was signifi-cantly higher than that in the control group from the 7th week(P<0.01).Compared with the control group,the levels of TG,TC and LDL-C in the model group were significantly increased(all P<0.01),and the level of HDL-C was significantly lower than that in the control group(P<0.05).Pathological observation showed that the volume of epididymal white fat in the model group was significantly increased.The abundance and diversity of intestinal flora in model group under the 16S rRNA analysis showed that the relative abundance of Firmicutes significantly increased,the relative abundance of Bacteroidetes significantly decreased,and the abundance of potential pathogenic bacteria such as Desulfobacterota also showed an upward trend.The contents of main SCFAs such as acetic acid,propionic acid and butyric acid in the feces of mice in the model group showed a continuous downward trend,and the contents were significantly lower than those in the control group starting from the 6th to 7th week(P<0.01).The contents of other trace SCFAs also showed a downward trend.The comparison of to-tal SCFAs content between the two groups showed that the model group was significantly lower than the control group since the 6th week(P<0.01).Conclusion The imbalance of SCFAs,a metabolite of intestinal flora,is related to the occurrence and development of obesity,mainly manifested as a significant decrease in the content of acetic acid,propionic acid and butyric acid,which in turn aggravates the patho-genesis of obesity.关键词
高脂饮食/肥胖/肠道菌群/短链脂肪酸/动态变化Key words
High-fat diet/Obesity/Intestinal flora/Short-chain fatty acids/Dynamic changes分类
医药卫生引用本文复制引用
程世姣,权浩洋,李甲,仲澳珍,吴海涛,郑敏..高脂饮食诱导肥胖小鼠模型中短链脂肪酸的动态观察[J].湖北科技学院学报（医学版）,2026,40(4):316-320,331,6.基金项目
咸宁市重点科技研发项目(2021SFYF003) （2021SFYF003）
湖北科技学院科研团队创新项目(2023T10) （2023T10）
湖北省智慧康养产业技术研究院专项(2022HX182) （2022HX182）
