畜牧与兽医2026，Vol.58Issue(9)：34-40,7.
低温应激对合作猪肝脏脂肪酸氧化代谢、PLIN5表达量及其互作网络作用研究
Low-temperature stress on liver fatty acid β-oxidation,PLIN5 expression and interaction networks in Hezuo pigs
摘要
Abstract
This study aimed to investigate the effects of cold stress on hepatic fatty acid oxidation metabolism,the expression of perilipin 5(PLIN5),and its interacting regulatory network in Hezuo pigs.Six healthy Hezuo pigs aged 75 days with similar body weights were chosen and randomly assigned to either a normal-temperature control group or a cold stress treatment group,with three biological replicates per group.After 24 h of treatment,the activities of key rate-limiting enzymes involved in fatty acid oxidation,including carnitine palmitoyltrans-ferase 1A(CPT1A)and medium-chain acyl-CoA dehydrogenase(ACADM),as well as free fatty acid(FFA)concentrations,were deter-mined using enzyme-linked immunosorbent assay(ELISA).The expression levels of PLIN5 were evaluated by reverse transcription quantita-tive PCR(RT-qPCR),Western blot,and immunohistochemistry.The protein-protein interaction network of PLIN5 was constructed using the STRING database,and the expression patterns of key interacting genes,including PLIN5,α/β-hydrolase domain-containing protein 5(ABHD5),adipose triglyceride lipase(ATGL),fatty acid-binding protein 4(FABP4),adiponectin(ADIPOQ),solute carrier family 2 member 4(SLC2A4),and uncoupling protein 2(UCP2),were detected by RT-qPCR.The results showed that cold stress significantly in-creased the enzymatic activities of CPT1A and ACADM in the liver of the Hezuo pigs(P＜0.05),markedly reduced hepatic FFA content(P＜0.01).The expression of PLIN5 was significantly upregulated at both the mRNA and protein levels after cold exposure(P＜0.05).The interaction network analysis revealed that PLIN5 interacted with 50 candidate genes.Among the key interacting genes,the expression levels of ABHD5,ATGL,and FABP4 were significantly increased(P＜0.01),whereas the expression levels ofADIPOQ,SLC2A4,and UCP2 were significantly decreased(P＜0.01).In conclusion,cold stress enhanced hepatic fatty acid oxidation metabolism in the Hezuo pigs by up-regulating PLIN5 expression and modulating the expression of its interacting molecules,thereby promoting heat production and improving cold adaptation.These findings highlighted the critical role of PLIN5 and its interaction network in the response to cold stress and provided valua-ble theoretical insights into the molecular mechanisms underlying cold tolerance in the liver of Hezuo pigs.关键词
合作猪/低温应激/肝脏/脂滴包被蛋白5Key words
Hezuo pig/cold stress/liver/PLIN5分类
农业科技引用本文复制引用
袁曼玲,高小莉,滚双宝..低温应激对合作猪肝脏脂肪酸氧化代谢、PLIN5表达量及其互作网络作用研究[J].畜牧与兽医,2026,58(9):34-40,7.基金项目
国家自然科学基金项目(U22A20507) （U22A20507）
岷山黑猪新品种选育及关键技术集成推广项目(22ZD6NA044) （22ZD6NA044）
甘肃农业大学畜牧学养猪产业技术创新团队项目(GAU-XKTD-2022-25) （GAU-XKTD-2022-25）
