山西农业大学学报（自然科学版）2026，Vol.46Issue(4)：91-100,10.DOI:10.13842/j.cnki.issn1671-8151.202601035
延胡索酸二钠对维氏气单胞菌KCL-1的抗菌活性及其毒力因子调控研究
Antibacterial activity of disodium fumarate against Aeromonas veronii KCL-1 and its regulatory effects on virulence factors
摘要
Abstract
[Objective]The purpose of current study was to investigate the in vitro antibacterial effects of disodium fumarate against Aeromonas veronii KCL-1 and its impact on the expression of virulence genes.[Methods]In this study,the minimum inhibitory concentration(MIC)of disodium fumarate against A.veronii KCL-1 was determined using the twofold serial dilu-tion method,and the minimum bactericidal concentration(MBC)was identified via the plate colony counting method.Growth curves of strain KCL-1 were plotted using the optical density method.Swarming and swimming assays were conducted to eval-uate the effect of disodium fumarate on bacterial motility.Furthermore,real-time quantitative PCR(RT-qPCR)was used to analyze the temporal expression profiles of key virulence genes in KCL-1 under disodium fumarate treatment.[Results]The MIC and MBC of disodium fumarate against KCL-1 were determined to be 1 mg/mL and 4 mg/mL,respectively.At a sub-in-hibitory concentration(0.5 mg/mL),disodium fumarate significantly inhibited biofilm formation,bacterial motility,and exo-polysaccharide synthesis.Meanwhile,RT-qRCR analysis revealed a down-regulation in the expression of key virulence fac-tors.These included exotoxin genes(hlyA,cylG,and rtxA),extracellular protease genes(lasB,orfM,and ilpA),adhesion factor genes(mshQ,flgD,and flhF),as well as secretion system related genes(esxA,ituA,and tapQ).Most of these viru-lence-related genes reached their lowest expression levels during the stationary phase(30 hours),synchronizing with the growth inhibition observed in the growth curve.[Conclusion]Disodium fumarate effectively inhibited the growth and motility of A.ve-ronii KCL-1 in a concentration dependent manner,while simultaneously down-regulating the expression of key virulence fac-tors in this strain.关键词
延胡索酸二钠/维氏气单胞菌/体外抑菌/毒力基因Key words
Disodium fumarate/Aeromonas veronii/In vitro antibacterial activity/Virulence gene分类
农业科技引用本文复制引用
徐振权,霍佳昕,韩卓然,孙敬锋..延胡索酸二钠对维氏气单胞菌KCL-1的抗菌活性及其毒力因子调控研究[J].山西农业大学学报（自然科学版）,2026,46(4):91-100,10.基金项目
天津市自然科学基金重点项目(24JCZDJC00450) （24JCZDJC00450）
