山西农业大学学报（自然科学版）2026，Vol.46Issue(4)：101-113,13.DOI:10.13842/j.cnki.issn1671-8151.202602015
β-羟基丁酸介导热应激子代社会行为改善机制
Mechanism of β-hydroxybutyrate-mediated improvement in social behavior deficits of heat-stressed offspring
摘要
Abstract
[Objective]This study aimed to elucidate the protective effects and underlying mechanisms of prenatal β-hydroxybu-tyrate(BHBA)supplementation on the neurodevelopment of heat-stressed offspring through systematic behavioral assessments combined with the evaluation of hippocampal neurogenesis indicator.[Methods]Pregnant mice were randomly assigned to three groups:control(CON),heat stress(HS),and BHBA intervention(BHBA+HS).From gestational days 10.5 to 18.5,mice in the BHBA+HS group received a daily intraperitoneal injection of 200 mg/(kg·d)BHBA,while the other groups were injected with an equivalent volume of physiological saline.One hour post-injection,the HS and BHBA+HS groups were ex-posed to 42 ℃ heat stress for 15 minutes.To evaluate changes across distinct stages of neurodevelopment,neural stem cell pro-liferation was detected on postnatal day 8(P8),newborn neuron survival,differentiation,and related gene expression were evaluated on P28,dendritic spine morphological analysis was conducted on P60.[Results]Behavioral assessments,including the open field test,elevated plus maze,and Morris water maze,demonstrated that BHBA significantly ameliorated social be-havior deficits in heat stressed offspring,confirming its neuroprotective efficacy.Molecular mechanism analysis revealed that maternal HS led to persistent social behavior deficits in offspring by disrupting dopaminergic signaling and synaptic integrity.Conversely,BHBA rescued the heat stress induced aberrant differentiation of neural stem cells into glial cells and promoted adult neurogenesis.These findings were further validated by alterations in hippocampal dendritic spine complexity and changes in the numbers of immunofluorolabeled newborn neurons.[Conclusion]This study confirmed that prenatal heat stress induced long-lasting neurobehavioral abnormalities in offspring by impairing hippocampal neurogenesis,disturbing dopaminergic signal-ing,and compromising synaptic plasticity.As an endogenous intervention,BHBA multi-targetingly promoted neurogenesis,corrected neurotransmitter imbalances,and remodeled synaptic structures,thereby restoring hippocampal neural circuit function and improving the behavioral phenotype of offspring.These findings not only provided novel insights into the mechanisms by which early-life environmental stress influences neurodevelopment but also offered a potential nutritional intervention strategy and a translational research framework for preventing and treating neurodevelopmental disorders associated with heat stress.关键词
β-羟基丁酸/宫内环境/热应激/行为障碍/神经发育Key words
Beta-hydroxybutyrate/Intrauterine environment/Heat stress/Behavioral disorders/Neurodevelopment分类
农业科技引用本文复制引用
张子君,赵婷婷,李亚杰,吕昊轩,卿素珠,朱晓岩..β-羟基丁酸介导热应激子代社会行为改善机制[J].山西农业大学学报（自然科学版）,2026,46(4):101-113,13.基金项目
国家自然科学基金(32272967 （）
32472994) （）
