畜牧与兽医2026，Vol.58Issue(9)：79-85,7.
布氏杆菌抗体磁微粒化学发光免疫分析方法的建立
Establishment of a magnetic particle chemiluminescent immunoassay method for Brucella antibodies
摘要
Abstract
To address the issues of insufficient accuracy and low efficiency in traditional brucellosis detection methods,this study aimed to establish a highly efficient and accurate antibody detection method for brucellosis.A magnetic particle-based chemiluminescent immunoassay(CLIA)for brucellosis antibody detection was established using smooth-type Brucella lipopolysaccharide(sLPS)-coated magnetic beads and acridinium ester-labeled Staphylococcus aureus protein A(SPA).This method was developed through optimization and threshold deter-mination.Its sensitivity,specificity,reproducibility,and stability were further validated,and it was subsequently applied to clinical sample testing.Testing of 250 clinical serum samples with known backgrounds established a cutoff value of 5 686 RLU.Performance validation dem-onstrated excellent specificity;the minimum detectable concentration for both ovine and bovine Brucella-positive sera was 1 IU/mL.The intra-and inter-batch coefficients of variation were both＜10%.The method maintained stable performance even when the serum samples had been stored for 15 months at 2-8℃.The method yielded accurate results with an overall agreement rate of 92.9%,compared with a commercially available Brucella antibody ELISA kit.The established detection method possessed advantages including simplicity,rapidity,high efficiency,and strong stability,providing a reliable technical approach for diagnosis and control of brucellosis.关键词
布氏杆菌病/磁微粒化学发光免疫分析/高通量抗体检测/临床应用Key words
brucellosis/magnetic particle chemiluminescence immunoassay/high-throughput antibody detection/clinical applications分类
农业科技引用本文复制引用
于海峰,钱艳红,陈晶,陈远媚,王晓虎,孙靖..布氏杆菌抗体磁微粒化学发光免疫分析方法的建立[J].畜牧与兽医,2026,58(9):79-85,7.基金项目
省级现代农业产业技术体系创新团队建设项目(2024CXTD15和2024CXTD15-01) （2024CXTD15和2024CXTD15-01）
广东省农村科技特派员重点派驻任务项目(KTP20240183) （KTP20240183）
广东省科技计划项目(2023A1111110001) （2023A1111110001）
2025年绿美广东生态建设重点任务保障(林业领域)专项 （林业领域）
广州市科技计划项目(2023B04J0137) （2023B04J0137）
广东省畜禽疫病防治研究重点实验室项目(2023B1212060040) （2023B1212060040）
