畜牧兽医学报2026，Vol.57Issue(8)：4363-4373,11.DOI:10.11843/j.issn.0366-6964.2026.08.022
种公鸡精液抗冻性状的全基因组关联分析
Genome-Wide Association Study of Semen Cryotolerance Traits in Roosters
摘要
Abstract
Based on genome-wide association study(GWAS)technology,this research aimed to identify single nucleotide polymorphisms(SNPs)and candidate genes associated with semen cryotolerance traits in roosters,in order to provide reference information for molecular breeding aimed at improving semen cryotolerance of roosters.A total of 215 healthy 65-week-old White Leghorn breeding roosters were selected as the experimental population.Fresh semen and frozen-thawed semen samples were collected,and freeze-tolerance indexes including variations in sperm motility,sperm viability and sperm abnormality rate were determined and calculated,with 3 biological replicates.Heritability was estimated using DMUAI software.Genomic DNA was extracted from wing vein blood samples,and genotyping was performed using the chicken 55K SNP array.After quality control with Plink v1.9 software,GWAS was conducted using the BLINK model embedded in GAPIT,and genetic effect analysis was carried out for SNP-linked candidate genes.The variation values of sperm motility and sperm viability presented low heritability ranging from 0.070 to 0.154,while the variation of sperm abnormality rate showed moderate heritability of 0.397.A total of 7 significant SNP loci were identified by GWAS.Three significant SNPs(chr6:31460756,chr24:4865901,chr24:5049139)associated with sperm viability variation were annotated to 3 candidate genes,namely CADM1,APOC3 and APOA1.Four significant SNPs(chr6:4901210,chr6:4902911,chr7:15775500,chr7:26741761)related to sperm abnormality rate variation corresponded to 4 candidate genes including DYDC2,MTX2,ADCY5 and MYLK.This GWAS on semen cryotolerance in White Leghorn roosters identified 7 key candidate genes,including APOA1,MTX2,and APOC3.The results provide valuable information for marker-assisted selection breeding aimed at improving semen cryotolerance in roosters.关键词
种公鸡/精液抗冻性/全基因组关联分析/单核苷酸多态性/候选基因Key words
rooster/semen cryotolerance/genome-wide association study(GWAS)/single nucleotide polymorphisms(SNPs)/candidate genes分类
农业科技引用本文复制引用
李欣欣,王德贺,陈一凡,郝二英,孙研研,孙二东,陈辉,石雷,李艳慧,宗云鹤,范毅豪,徐俊杰,吴星霖,姚懿纯,张荣财,李浩瑜..种公鸡精液抗冻性状的全基因组关联分析[J].畜牧兽医学报,2026,57(8):4363-4373,11.基金项目
保定市科技计划项目(2394N012) （2394N012）
保定市"揭榜挂帅"项目(2023创127) （2023创127）
保定市"揭榜挂帅"项目(2024农101 （）
2024农206) （）
河北省现代农业产业技术体系建设项目(HBCT2024260201) （HBCT2024260201）
