畜牧与兽医2026，Vol.58Issue(9)：103-113,11.
基于网络药理学和转录组学探讨马齿苋抗猪流行性腹泻病毒的作用机制
Exploring the mechanism of Portulaca oleracea L.against porcine epidemic diarrhea virus based on network pharmacology and transcriptomics
摘要
Abstract
This study aimed to investigate the mechanisms by which Portulaca oleracea L.inhibits porcine epidemic diarrhea virus(PEDV)replication through transcriptomics and network pharmacology,and to validate its key active components.A PEDV-infected Vero cell model was established,and RNA-seq analysis was performed to identify differentially expressed genes(DEGs)following purslane treatment,fol-lowed by functional enrichment analysis.Concurrently,network pharmacology was employed to screen active ingredients and their targets,construct a protein-protein interaction(PPI)network,identify core genes,and conduct molecular docking.In vitro experiments were carried out to evaluate the effects of quercetin on cytotoxicity,viral replication,lactate dehydrogenase(LDH)release,ATP synthesis,and cell sur-vival rate.Compared with the virus group,1 425 DEGs were identified in the purslanetreated group,of which 584 were reversely expressed genes,mainly enriched in the TNF,apoptosis,and MAPK pathways.Network pharmacology yielded 10 active ingredients and 111 targets;the PPI network identified seven core genes,including EGFR,ESR1,and TP53,among which quercetin was associated with the most targets and exhibited good binding affinity to these core targets.In vitro experiments confirmed that quercetin had no significant cytotoxicity below 19.3 μg/mL,and dosedependently inhibited LDH release,ATP decline,and viral replication while improving cell survival rate.In conclu-sion,P.oleracea may exert its antiPEDV effects through synergistic regulation of apoptosis and inflammatory pathways by multiple compo-nents,including quercetin,providing a basis for the development of antiviral drugs.关键词
马齿苋/网络药理学/转录组学/猪流行性腹泻病毒/活性成分Key words
Portulaca oleracea L./network pharmacology/transcriptomics/PEDV/active ingredients分类
农业科技引用本文复制引用
张雨林,林树乾,闫遵祥,张瑜,殷斌,杨世发,赵增成,刘月月,孙爱军..基于网络药理学和转录组学探讨马齿苋抗猪流行性腹泻病毒的作用机制[J].畜牧与兽医,2026,58(9):103-113,11.基金项目
国家自然科学基金项目(32302918,32302919) （32302918,32302919）
国家重点研发项目子课题项目(2023YFD1800804-06) （2023YFD1800804-06）
山东省科技型中小企业创新能力提升工程项目(2023TSGC0006) （2023TSGC0006）
山东省重点研发计划项目(2023TZXD083) （2023TZXD083）
