水生生物学报2026，Vol.50Issue(9)：95-107,13.DOI:10.3724/1000-3207.2026.2026.0071
马口鱼性别分化与XY伪雌鱼的诱导及其繁殖研究
SEX DIFFERENTIATION,INDUCTION OF XY PSEUDO-FEMALE AND THEIR REPRODUCTION IN HOOKSNOUT CARP(OPSARIICHTHYS BIDENS)
摘要
Abstract
The hooksnout carp(Opsariichthys bidens)exhibits pronounced sexual dimorphism in growth,with males attaining a growth rate more than 1.5 times that of females.In addition,males develop distinctive nuptial color stripes,which enhance their visual recognition and ornamental value.As such,the production of all-male populations holds significant potential for improving aquaculture efficiency.To elucidate the biological mechanisms underlying sex differentiation and to establish a technological system for generating all-male offspring,this study first systematically characterized the sexual dimorphism of the species through observation of natural populations.Histological analysis was then employed to track the continuous developmental process from hatching through the completion of sex diffe-rentiation,identifying the critical period for sex differentiation as occurring between 35 and 100 days post-hatching(dph).Accordingly,exogenous sex reversal was induced via dietary administration of 100 mg/kg 17β-estradiol(E2)from 25 to 70 dph.Following treatment,gonadal phenotypes comprised ovaries(86.0％),intersex gonads(11.1％),and testes(2.9％),confirming the successful production of fertile XY pseudo-females.Subsequent crosses between these XY pseudo-females and normal XY males successfully generated YY super-males.This study systematically defines the critical period and histological characteristics of sex differentiation in hooksnout carp,demonstrates the efficacy of dietary E2 administration in inducing sex reversal in XY individuals,and establishes a foundational genetic breeding pathway from XY pseudo-females to YY super-males.These findings provide both a theoretical framework and a tech-nical basis for the development of a novel all-male germplasm in hooksnout carp.关键词
性别分化/性别二态性/17β-雌二醇/伪雌鱼/超雄鱼/种质创制/马口鱼Key words
Sex differentiation/Sexual dimorphism/17β-estradiol/Pseudo-female/Super-male/Germplasm creation/Opsariichthys bidens分类
农业科技引用本文复制引用
陈玉佩,任旌雷,杨文锐,陈国浩,邓翔,樊启学,何焱,沈志刚..马口鱼性别分化与XY伪雌鱼的诱导及其繁殖研究[J].水生生物学报,2026,50(9):95-107,13.基金项目
湖北省支持种业高质量发展资金(HBZY2023B009) （HBZY2023B009）
湖北省技术创新计划(2024BBB016)资助[Supported by Hubei's Project Funding for Supporting High-Quality Development of the Seed Industry(HBZY2023B009) （2024BBB016）
Hubei Technological Innovation Program Project(2024BBB016)] （2024BBB016）
