畜牧兽医学报2026，Vol.57Issue(8)：4540-4549,10.DOI:10.11843/j.issn.0366-6964.2026.08.038
杨梅素抑制非洲猪瘟病毒感染猪肺泡巨噬细胞作用的鉴定
Identification of the Inhibitory Effect of Myricetin on African Swine Fever Virus Infection in Porcine Alveolar Macrophages
摘要
Abstract
African swine fever(ASF),caused by the African swine fever virus(ASFV),has inflicted substantial losses on the swine industry in our country.Flavonoids exhibit broad-spectrum antiviral activities.Myricetin,a type of flavonoid,has demonstrated antiviral activities against various RNA/DNA viruses.This study evaluates the role of myricetin against genotype II ASFV infectivity to porcine alveolar macrophages(PAMs).During the infection of PAMs by ASFV,which were treated with varying concentrations of myricetin.The impacts of myricetin on the infectivity of ASFV to PAMs were evaluated using methods such as real-time quantitative PCR,Western blotting,immunofluorescence,and hemadsorption assays.Myricetin exhibits minimal toxicity to PAMs,with a CC50 value of(152.270±1.091)μmol·L-1;Myricetin can significantly reduce the viral yield of ASFV,with an IC50 value of(31.670±1.489)μmol·L-1,and it markedly inhibits the transcription of the viral early gene CP204L and the expression of its encoded protein p30;The inhibitory effect is most significant when myricetin is used to pretreat the virus and when added during the virus invasion of PAMs,whereas pretreatment of PAMs with myricetin and addition of myricetin post ASFV infection of PAMs have no significant impact on the viral yield of ASFV;Further results show that myricetin significantly inhibits the transcription of the viral early gene CP204L and the expression of its encoded protein p30 during the adhesion and internalization of ASFV into PAMs.This study is the first to reveal that myricetin exhibits a certain inhibitory effect on ASFV infection in PAMs.关键词
非洲猪瘟病毒/杨梅素/猪肺泡巨噬细胞/阻断/入侵Key words
African swine fever virus/myricetin/porcine alveolar macrophages/blockade/invasion分类
农业科技引用本文复制引用
王琼洁,关贵全,田占成,牛庆丽,独军政,赵亚茹,杨赛霞,杨吉飞,张忠辉,殷宏..杨梅素抑制非洲猪瘟病毒感染猪肺泡巨噬细胞作用的鉴定[J].畜牧兽医学报,2026,57(8):4540-4549,10.基金项目
甘肃省自然科学基金项目(25JRRA436) （25JRRA436）
甘肃省科技发展重大专项(22ZD6NA001) （22ZD6NA001）
中央公益性科研院所基本科研业务费专项(CAAS-ZDRW202409) （CAAS-ZDRW202409）
中国农业科学院农业科技创新工程项目(CAAS-ASTIP-2021-LVRI) （CAAS-ASTIP-2021-LVRI）
