水生生物学报2026，Vol.50Issue(9)：108-117,10.DOI:10.3724/1000-3207.2026.2026.0124
二十碳五烯酸对大菱鲆肌肉原代细胞中蛋白质周转和代谢的影响
EICOSAPENTAENOIC ACID ON PROTEIN TURNOVER AND METABOLISM IN THE PRIMARY MUSCLE CELLS OF TURBOT(SCOPHTHALMUS MAXIMUS)
摘要
Abstract
Eicosapentaenoic acid(EPA)is known to influence skeletal muscle protein turnover in mammals,but its effect on teleost and the underlying mechanisms remain unclear.In this study,the impact of EPA on cellular protein accumulation and metabolism of lipid and glucose were examined in turbot muscle cells.The nascent protein synthesis and the activities of the protein kinase B(Akt)/the mechanistic target of rapamycin(mTOR)signaling pathway were enhanced by EPA.Furthermore,EPA repressed the expression of Atrogin-1 and Muscle Ring Finger protein-1(MuRF-1),two dominant muscle-specific ubiquitin ligases.EPA also shifted cellular metabolism,as reflected by changes in the expression of key enzymes involved in lipid metabolism and glycolysis.These results suggested that EPA might exert beneficial effects on protein turnover by stimulating protein synthesis,repressing ubiquitin-proteasome pathway-media-ted protein degradation,as well as reprogramming cellular metabolism.Our findings support that the function of EPA on promoting protein accumulation is conserved in teleost.关键词
二十碳五烯酸(EPA)/Akt/mTOR信号通路/糖脂代谢/蛋白质沉积/蛋白质周转/大菱鲆Key words
Eicosapentaenoic acid(EPA)/Akt/mTOR signaling pathway/Glucose and lipid metabolism/Protein accre-tion/Protein turnover/Turbot分类
农业科技引用本文复制引用
崔瑾,侯宜颖,刘成栋,周慧慧,王旋,麦康森,何艮..二十碳五烯酸对大菱鲆肌肉原代细胞中蛋白质周转和代谢的影响[J].水生生物学报,2026,50(9):108-117,10.基金项目
山东省自然科学基金(ZR2025MS495)资助[Supported by Natural Science Foundation of Shandong Province(ZR2025MS495)] （ZR2025MS495）
