天津科技大学学报2026，Vol.41Issue(4)：1-9,9.DOI:10.13364/j.issn.1672-6510.20250013
阪崎克罗诺杆菌基因clsA功能及耐干燥机制研究
Function and Desiccation Mechanism of Gene clsA in Cronobacter sakazakii
摘要
Abstract
Cronobacter sakazakii exhibits strong desiccation resistance;however,the underlying mechanisms remain un-clear.Previous studies using a random mutation library method identified that the cardiolipin synthase gene clsA significantly affected desiccation resistance.In order to further explore the role of clsA in the desiccation resistance process of Cronobac-ter sakazakii,a mutant strain ΔclsA was constructed by homologous recombination method,and a complementation strain cpclsA was generated by vector pACYC184.Comparing the physiological characteristics of the wild-type(WT),ΔclsA and cpclsA strains,it was found that the deletion of clsA significantly reduced the content of cardiolipin by 38.6%,and the desic-cation inactivation rate increased from(50.73±2.91)%to(66.40±3.30)%.Quantitative real-time PCR(qRT-PCR)and specific metabolite content analysis showed that the deletion of gene clsA led to a decrease of about 24.0%in proline content and a decrease in betaine content of about 31.4%,which may be the main reason that gene clsA caused the reduced desicca-tion resistance of the mutant strain.Furthermore,the motility,biofilm formation ability,and surface hydrophobic properties of the mutant strain was also significantly reduced.This study contributes to a better understanding of the function and mechanism of clsA in the desiccation resistance process of Cronobacter sakazakii.关键词
阪崎克罗诺杆菌/耐干燥/心磷脂合成/clsA基因Key words
Cronobacter sakazakii/desiccation resistance/cardiolipin synthesis/clsA gene分类
轻工纺织引用本文复制引用
丁子怡,曹娅菲,司默涵,李萍,杜欣军..阪崎克罗诺杆菌基因clsA功能及耐干燥机制研究[J].天津科技大学学报,2026,41(4):1-9,9.基金项目
国家自然科学基金面上项目(32472322) （32472322）
