天津科技大学学报2026，Vol.41Issue(4)：10-16,80,8.DOI:10.13364/j.issn.1672-6510.20240256
外源γ-氨基丁酸对PCPA诱导焦虑失眠小鼠脑内Glu/GABA平衡的影响分析
Analysis on the Effects of Exogenous γ-Aminobutyric Acid on Glu/GABA Homeostasis in the Brain of PCPA-Induced Anxiety-Insomnia Mice
摘要
Abstract
This study aimed to investigate the mechanism of exogenous γ-aminobutyric acid(GABA)on the metabolic balance of Glu/GABA in the brain of p-chlorophenylalanine(PCPA)-induced anxiety-insomnia mice.Sixty female Kunming mice were selected to create a mouse model of insomnia and anxiety by intraperitoneal injection of PCPA for 2 consecutive days.Behavioural tests were carried out to detect changes in physicochemical indexes,pathological changes in hippocampus and changes in the relative abundance of mRNA of GABRA-3 and GABRA-5 in the brain tissue of mice.The results showed that compared with the model group,after the administration of high-dose GABA,the anaesthesia induction time of mice was significantly reduced(P＜0.000,1),the number of shuttles in the bright and dark boxes and the stay time in the dark room were significantly reduced(P＜0.000,1),the contents of 5-HT(P＜0.000,1)and GABA(P＜0.01)in the brain were increased and the content of Glu(P＜0.01)was down-regulated.Additionally,the brain neuronal cells of the mice were restored,the contents of brain-derived neurofactors were elevated(P＜0.000,1),and the mRNA levels of GABRA-3 and GABRA-5 in the brain were elevated(P＜0.05,P＜0.001).Therefore,a certain dose of exogenous GABA has a sedative-hypnotic effect,and its mechanism of action may be related to increasing the levels of GABA and 5-HT in the mouse brain,regulating the nervous system,ameliorating the damage of mouse hippocampal neuronal cells,up-regulating the mRNA levels of the receptors GABRA-3 and GABRA-5,and regulating the balance of the Glu/GABA metabolism.关键词
外源γ-氨基丁酸/5-HT/Glu/GABA/GABRA/海马体Key words
exogenous γ-aminobutyric acid/5-HT/Glu/GABA/GABRA/hippocampus分类
轻工纺织引用本文复制引用
霍超,敬晓冬,王怡宁,孙志静,罗学刚..外源γ-氨基丁酸对PCPA诱导焦虑失眠小鼠脑内Glu/GABA平衡的影响分析[J].天津科技大学学报,2026,41(4):10-16,80,8.基金项目
天津市科技成果转移转化项目(24ZYCGSY00390) （24ZYCGSY00390）
