湖北科技学院学报（医学版）2026，Vol.40Issue(4)：295-300,6.DOI:10.16751/j.cnki.2095-4646.2026013010
毛蕊花糖苷抗胶质母细胞瘤作用机制研究
Study on the Mechanism of Action of Acteoside Against Glioblastoma
摘要
Abstract
Objective To screen the potential targets of Acteoside(ACT)against glioblastoma based on network pharmacology and molecular docking technology,and to explore its inhibitory effect on malignant progression and the underlying molecular mechanisms through in vitro experiments.Methods The component targets of ACT and disease-related targets of glioblastoma were first retrieved from databases to construct a drug-target-disease visualization network and a protein-protein interaction(PPI)network,followed by GO and KEGG enrichment analyses.Molecular docking was performed to validate potential interaction targets.Cell proliferation activity was assessed by CCK-8 assay,cell migration and invasion abilities were examined using Transwell assay,and Western blot was employed to detect the expression changes of key MAPK pathway proteins(ERK,p-ERK)and downstream effector molecules(MMP2,MMP9).Furthermore,a rescue experiment using the MAPK pathway agonist EGF was conducted to verify the functional role of the pathway in the action of ACT.Results Network pharmacol-ogy and molecular docking suggested that MMP2 and MMP9 are potential key targets of ACT in glioblastoma.In vitro experiments showed that ACT inhibited glioblastoma cell viability,migration,and invasion,and down-regulated the expression of p-ERK,MMP2,and MMP9 pro-teins.Moreover,the MAPK pathway agonist EGF reversed the inhibitory effects of ACT on the expression of these proteins and the associated cellular phenotypes.Conclusion ACT may inhibit the proliferation,migration,and invasion of glioblastoma cells by suppressing the MAPK signaling pathway and downregulating the expression of MMP2 and MMP9.This study systematically elucidates the mechanism of action from computational prediction to experimental validation,providing a theoretical basis for the development of ACT as an anti-glioblastoma drug.关键词
毛蕊花糖苷/胶质母细胞瘤/侵袭/网络药理学/MAPK通路Key words
ACT/Glioblastoma/Invasion/Network pharmacology/MAPK pathway分类
医药卫生引用本文复制引用
尹鹏翔,蒋赛男,马婉璐,杨洁,陈洪国..毛蕊花糖苷抗胶质母细胞瘤作用机制研究[J].湖北科技学院学报（医学版）,2026,40(4):295-300,6.基金项目
湖北省自然科学基金项目(2024AFB1057,2021BBA098) （2024AFB1057,2021BBA098）
