天津科技大学学报2026，Vol.41Issue(4)：40-48,9.DOI:10.13364/j.issn.1672-6510.20240240
漆酶与木聚糖酶作用下木质素-碳水化合物复合体的降解及其生物活性
Degradation and Biological Activity of Lignin-Carbohydrate Complex by Laccase and Xylanase
摘要
Abstract
Using lignin carbohydrate complex(LCC)extracted from eucalyptus pre-hydrolysis solution and lignin samples rich in LCC fragments(LCC-AcOH)as raw materials,the changes in component content were investigated after treatment with different amounts of laccase and xylanase,and a series of characterizations and biological activities were explored after enzyme treatment.The results showed that the lignin mass concentration of the degradation solutions of LCC and LCC-AcOH treated with a laccase dosage of 3.2 U/mL decreased the most,by 12.70%and 12.94%respectively.The total sugar mass concentration of the degradation solutions of LCC and LCC-AcOH treated with laccase dosages of 1.6 U/mL and 2.4 U/mL decreased the most,by 5.98%and 33.98%respectively.When treated with a xylanase dosage of 1 200 U/mL,the lignin mass concentration of the degradation solutions of LCC and LCC-AcOH increased the most,by 30.95%and 10.45%respectively.When treated with a xylanase dosage of 800 U/mL,the total sugar mass concentration of the degradation solu-tions of LCC and LCC-AcOH increased the most,by 69.80%and 73.44%respectively.After enzymatic treatment,LCC and LCC-AcOH treated with laccase dosages of 1.6 U/mL and 0.8 U/mL,as well as LCC and LCC-AcOH treated with xylanase dosages of 1 200 U/mL and 300 U/mL respectively,had the strongest antioxidant and antibacterial abilities.These results indicate that the enzymatic hydrolysis of LCC shows great promise in the application of LCC.关键词
木质素-碳水化合物复合体(LCC)/漆酶/木聚糖酶/降解Key words
lignin carbohydrate complex(LCC)/laccase/xylanase/degradation分类
化学化工引用本文复制引用
王茜,刘盛,廖小元,刘海棠..漆酶与木聚糖酶作用下木质素-碳水化合物复合体的降解及其生物活性[J].天津科技大学学报,2026,41(4):40-48,9.基金项目
国家自然科学基金项目(31700516) （31700516）
