湖北医药学院学报2026，Vol.45Issue(4)：409-413,封2,封3,7.DOI:10.13819/j.issn.2096-708X.2026.04.001
人胚胎干细胞来源的肠类器官移植发育后组织细胞来源解析
Analysis of Cellular Origins in Transplanted Intestinal Organoids Derived from Human Embryonic Stem Cells
摘要
Abstract
Objective To investigate the origins of various cell types in human embryonic stem cell(hESC)-derived intesti-nal organoids following transplantation and maturation in immunocompetent mice.Methods Three-dimensional intestinal or-ganoids were generated via directed differentiation of hESCs and transplanted beneath the kidney capsule of wild-type R26-EGFP mice.EGFP fluorescence tracing and immunofluorescence staining were employed to analyze the sources of different cell types after in vivo development of intestinal organoids.Results Following transplantation,the organoids survived and underwent further maturation.Fluorescence tracing and immunofluorescence staining revealed that smooth muscle cells and immune cells were primarily derived from the host,while neuronal cells and interstitial cells of Cajal originated mainly from the transplanted organoids.Vascular cells were found to originate from both the host and the organoids.Conclusion Human intestinal organoids can successfully engraft and differentiate in immunocompetent hosts,and different tissue layers have distinct cellular origins.This study lays a foundation for further mechanistic research and drug development using intestinal organoids.关键词
胚胎干细胞/肠类器官/肾包膜移植/细胞来源Key words
Embryonic stem cells/Intestinal organoids/Kidney capsule transplantation/Cellular origin引用本文复制引用
慕文博,庹鹏,胡翊寒,王小莉..人胚胎干细胞来源的肠类器官移植发育后组织细胞来源解析[J].湖北医药学院学报,2026,45(4):409-413,封2,封3,7.基金项目
"十四五"湖北省高等学校优势特色学科群(现代医学)项目(2024XKQY52,2025XKQY51) （现代医学）
湖北省脐带血造血干细胞治疗临床医学研究中心开放课题(2025SCOF013) （2025SCOF013）
研究生科技创新项目(YC202508) （YC202508）
湖北医药学院教研项目(YHJ2022041) （YHJ2022041）
