湖北医药学院学报2026，Vol.45Issue(4)：479-485,495,8.DOI:10.13819/j.issn.2096-708X.2026.04.013
CDK10 在鳞状细胞癌中的表达及功能分析
Expression and Functional Analysis of CDK10 in Squamous Cell Carcinoma
摘要
Abstract
Objective To explore the expression,prognostic value,and functional effects of CDK10 in squamous cell carci-noma(SCC),as well as its underlying molecular mechanisms.Methods GEPIA2 and STRING databases were used to ana-lyze the differential expression of CDK10 across pan-cancer types,clarify its prognostic value in head and neck squamous cell carcinoma(HNSCC),and construct a protein-protein interaction(PPI)network.A431 cells were cultured in vitro and divided into a negative control group and two siRNA knockdown groups(siRNA-876 and siRNA-1000).The knock-down efficiency was confirmed by qPCR and Western blot.Changes in cell proliferation,migration,and apoptosis were e-valuated using high-content imaging,wound healing assay,and flow cytometry.Transcriptome sequencing,as well as GO and KEGG enrichment analysis,was performed for the knockdown and control groups.Results CDK10 expression was dys-regulated in various tumors,including HNSCC,and its elevated level was significantly correlated with poor disease-free survival.Knockdown of CDK10 using siRNA-1000 effectively suppressed its expression in A431 cells,leading to attenuated proliferation,reduced migration by an approximately 50%(P＜0.01),and increased apoptosis(P＜0.01).RNA-seq anal-ysis identified 460 differentially expressed genes,which were predominantly enriched in pathways related to amino acid me-tabolism,DNA replication and repair,and glutathione metabolism.Conclusion CDK10 is overexpressed in HNSCC,which is associated with unfavorable prognosis.Knockdown of CDK10 can inhibit proliferation and migration and induce apoptosis in A431 cells,possibly through mechanisms involving metabolism,DNA repair,and cell cycle regulation.关键词
CDK10/鳞状细胞癌/A431 细胞/细胞增殖/细胞迁移/凋亡Key words
CDK10/Squamous cell carcinoma(SCC)/A431 cells/Cell proliferation/Cell migration/Apoptosis引用本文复制引用
李征远,常蓓蕾,尚杨,王悦,李贤玉..CDK10 在鳞状细胞癌中的表达及功能分析[J].湖北医药学院学报,2026,45(4):479-485,495,8.基金项目
湖北省科技厅基础研究类平台项目(2025CSA102) （2025CSA102）
湖北医药学院人才启动金项目(2021QDJZR007) （2021QDJZR007）
