湖北医药学院学报2026，Vol.45Issue(4)：486-492,7.DOI:10.13819/j.issn.2096-708X.2026.04.014
新藤黄酸纳米脂质体的构建与抗肝癌性能评价
Construction of Gambogenic Acid Nanoliposomes and Evaluation of Their Anti-hepatocellular Carcinoma Activity
摘要
Abstract
Objective To prepare liposomes loaded with gambogenic acid(GNA@Lip)and explore the in vitro anti-hepato-cellular carcinoma mechanism of GNA@Lip.Methods ⑴GNA@Lip was characterized in terms of appearance,particle size distribution,zeta potential,encapsulation efficiency,drug loading,and in vitro drug release.⑵Proliferation and apop-tosis assays were performed to evaluate the growth-inhibitory and apoptosis-inducing activities of GNA@Lip against hepato-cellular carcinoma cells.⑶Transwell assays were conducted to assess the anti-invasion capability of GNA@Lip.⑷Its effects on mitochondrial membrane potential and reactive oxygen species(ROS)accumulation in hepatocellular carcinoma cells were examined.Results ⑴TEM morphology observation revealed that GNA@Lip exhibited a spherical shape,with an average particle size and zeta potential of(103.4±1.14)nm and(-19.90±1.53)mV,respectively.Its encapsulation effi-ciency reached(91.45±1.26)%,and the drug loading capacity was(5.31±0.97)%.The in vitro release curve indicated that GNA@Lip had a sustained-release effect.⑵GNA@Lip demonstrated high safety for human normal liver cells(THLE-2),with the IC50 values against HepG2,Huh7,and Hep3B being 3.62,4.65,and 5.07 μmol/L,respectively,which were 0.46,0.54,and 0.59 times those of gambogenic acid(GNA)solution(IC50=7.94,8.59,and 8.58 μmol/L),re-spectively,showing pronounced growth-inhibitory effects.⑶Compared with GNA solution,GNA@Lip exerted significantly stronger pro-apoptotic and anti-invasion effects on HepG2 cells.(4)GNA@Lip effectively induced mitochondrial mem-brane potential depolarization and intracellular ROS accumulation,suggesting that GNA@Lip-medicated apoptosis in HepG2 cells may involve the mitochondrial pathway.Conclusion This study successfully prepares GNA@Lip,which can enhance the in vitro anti-hepatocellular carcinoma activity of GNA,providing a novel nanodelivery strategy to improve the efficacy of GNA against hepatocellular carcinoma.关键词
新藤黄酸/脂质体/表征/抗肿瘤/肝癌Key words
Gambogenic acid/Liposome/Characterization/Antitumor/Hepatocellular carcinoma引用本文复制引用
郭春燕,付卫争..新藤黄酸纳米脂质体的构建与抗肝癌性能评价[J].湖北医药学院学报,2026,45(4):486-492,7.基金项目
安徽省高校自然科学重大研究项目(2024AH040232) （2024AH040232）
