中国动物检疫2026，Vol.43Issue(7)：113-120,8.DOI:10.3969/j.issn.1005-944X.2026.07.016
重组猪干扰素-α冻干制备及体外抗病毒活性研究
Preparation of Lyophilized Recombinant Porcine Interferon-α and Its in vitro Anti-viral Activity
摘要
Abstract
Porcine interferon-α(poIFN-α)is recognized as a cytokine with antiviral activity.In order to study its potential value as a therapeutic agent for the prevention and control of swine viral diseases,a recombinant baculovirus capable of secreting recombinant poIFN-α(rpoIFN-α)was successfully constructed using the Bac-to-Bac baculovirus expression system,and the secretory expression of rpoIFN-α in vitro was achieved using SF9 insect suspension cell cultures.Subsequently,the optimal expression parameters were determined through optimizing the multiplicity of infection(MOI)of recombinant baculovirus,the expression duration post-infection,and in combination with the activity assay of rpoIFN-α against vesicular stomatitis virus(VSV)in harvested cell culture supernatants.The results showed that the antiviral activity of the obtained rpoIFN-α reached a peak(1.0× 107 IU/mL)when SF9 suspension cells were infected with the recombinant baculovirus at passage 4 at an MOI of 0.01 for 6 days;the purified rpoIFN-αwas obtained with a concentration of 0.58 mg/mL and a purity of 96.3％;and the lyophilized formulation of rpoIFN-αpresented a uniform milky white appearance with smooth surface and no collapse,and after lyophilization,its antiviral activity against VSV was not lost.The industrial development of poIFN-α antiviral biologics was supported by the data obtained in the study.关键词
猪干扰素-α/SF9细胞/蛋白纯化/抗病毒活性/冻干Key words
poIFN-α/SF9 cell/protein purification/antiviral activity/lyophilization分类
农业科技引用本文复制引用
杨文静,韩姗姗,闫锦初,周文,张瑞丽,戴荣莲,夏庆祥,秦立廷,吕闯..重组猪干扰素-α冻干制备及体外抗病毒活性研究[J].中国动物检疫,2026,43(7):113-120,8.基金项目
山东省重点研发计划(竞争性创新平台)项目(2023CXPT059) （竞争性创新平台）
