郭明佳 1赵梦姣 2孙圣福 3王浚宇 1李通 1李瑞丽 4曹积武 1郑孟加3
作者信息
- 1. 龙口市动物疫病预防控制中心,山东龙口 265701
- 2. 烟台市动物疫病预防与控制中心,山东烟台 264000
- 3. 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心),山东省人兽共患病重点实验室,山东济南 250101
- 4. 烟台市农业科学研究院,山东烟台 265500
- 折叠
摘要
Abstract
In order to systematically analyze the structural characteristics and biological functions of the 073 protein of Orf virus(ORFV),and to provide a basis for developing novel vaccine formulations,establishing specific detection methods,and elucidating the molecular mechanisms of viral immune evasion,the molecular features of the 073 protein were systematically predicted and analyzed using bioinformatics tools,including its physicochemical properties,hydrophilic-hydrophobic characteristics,functional domains,secondary structure,and tertiary conformation.Bioinformatics analysis revealed that the 073 protein consisted of 188 amino acid residues,with a theoretical molecular weight of 21.8 kDa.The protein was classified as unstable(instability index＞40),with a theoretical isoelectric point of 11.98,indicating a strongly basic nature.Hydrophilicity analysis showed that the grand average of hydropathicity(GRAVY)was-0.46,indicating that the protein was highly hydrophilic.Secondary structure prediction showed that α-helices accounted for 50.00％,random coils for 42.55％,and β-sheets for only 7.45％,indicating a typical α-helix-dominant structure.Subcellular localization prediction revealed a high probability of nuclear localization(52.2％),followed by mitochondrial localization(34.8％).A total of 15 potential phosphorylation sites were identified,including 8 serine residues,5 threonine residues,and 2 tyrosine residues.In conclusion,the 073 protein was basic and hydrophilic,with typical structural features characterized by instability,a tendency toward nuclear localization,and numerous potential phosphorylation sites,which might be closely associated with its key functions in the viral life cycle.A molecular basis was provided by this study for further understanding the pathogenic mechanisms of ORFV,and for developing new control strategies.关键词
羊口疮病毒/073蛋白/生物信息学/预测分析Key words
ORFV/073 protein/bioinformatics/predictive analysis分类
农业科技
