中国兽医科学2026，Vol.56Issue(8)：1042-1048,7.DOI:10.16656/j.issn.1673-4696.2026.0143
基于PCV2骨架的PCV3 Cap嵌合病毒反向遗传系统构建及免疫原性评价
Construction of a PCV3 Cap chimeric virus reverse genetics system based on PCV2 backbone and evaluation of its immunogenicity
摘要
Abstract
Porcine circovirus type 3(PCV3)is an emerging pathogen posing a significant threat to the swine industry.However,the isolation and cultivation of native PCV3 are extremely challenging,and obtaining the natural virus remains difficult,which severely hinders research into its pathogenic mechanisms and the development of vaccines.To address this core bottleneck,this study adopted an innova-tive approach to construct a recombinant PCV3 using reverse genetics technology.Given the well-estab-lished reverse genetics system and clear genetic background of PCV2,a chimeric viral genomic clone,pSK-PCV2-3,was successfully constructed by replacing the capsid protein(Cap)gene of a PCV2 infectious clone with the chemically synthesized PCV3 Cap gene via homologous recombination.Following transfection of this plasmid into Vero cells,an infectious chimeric virus,PCV2-3,was successfully rescued.Serial passaging and molecular characterization confirmed that the rescued virus maintained genetic stability in Vero cells and correctly expressed the PCV3 Cap protein.Purified virus particles exhibited a diameter of approximately 17 nm,a morphology consistent with the typical characteristics of circoviruses.Animal studies demonstrated that immunization of BALB/c mice with PCV2-3 induced high levels of PCV3 Cap-specific antibodies and significantly promoted splenic lymphocyte proliferation and the secretion of Th1-type cytokines(IFN-γ,TNF-α).This study reports the first successful establishment of a reverse genetics system for a PCV3 chimeric virus based on the PCV2 backbone,overcoming the technical challenge of obtaining native PCV3 and providing a critical technological platform and candidate material for research into PCV3 pathogenesis and vaccine development.关键词
PCV3/反向遗传系统/嵌合病毒/免疫原性Key words
PCV3/reverse genetics system/chimeric virus/immunogenicity分类
农业科技引用本文复制引用
赵范杰,李毅,黄晋炜,魏衍全,孙世琪,郭慧琛,白满元..基于PCV2骨架的PCV3 Cap嵌合病毒反向遗传系统构建及免疫原性评价[J].中国兽医科学,2026,56(8):1042-1048,7.基金项目
兰州市青年科技人才创新项目(2024-QN-3) （2024-QN-3）
国家重点研发计划项目(2023YFD1800300) （2023YFD1800300）
甘肃省重点研发计划项目(25YFNA014) （25YFNA014）
甘肃省技术创新引导计划-东西部科技协作专项(24CXNA030) （24CXNA030）
甘肃省科技重大专项(24ZD-WA004,23ZDNA007) （24ZD-WA004,23ZDNA007）
甘肃省省级人才项目-青年团队项目(2025QNTD34) （2025QNTD34）
甘肃省科技计划项目(24JRRA012,25JRRA442) （24JRRA012,25JRRA442）
