中国兽医科学2026，Vol.56Issue(8)：1058-1066,9.DOI:10.16656/j.issn.1673-4696.2026.0150
猫杯状病毒VP1-CDE蛋白单链抗体的筛选与鉴定
Screening and identification of single-chain fragment variable against VP1-CDE protein of feline calicivirus
摘要
Abstract
To develop novel diagnostic and therapeutic tools against feline calicivirus(FCV),phage display technology was employed to screen single-chain fragments variable(scFvs)that specifically target the conserved CDE region of capsid protein VP1(VP1-CDE)of FCV.First,the VP1-CDE protein was produced through recombinant expression and used to immunize SPF chickens,followed by the construc-tion of a chicken-derived scFv phage display library.After three rounds of biopanning,three highly specific scFvs,designated P2D5,P2A7,and PlG9,were successfully obtained.ELISA and Western-blot analyses demonstrated that these scFvs specifically recognized the recombinant VP1-CDE protein with-out cross-reactivity to unrelated proteins.Furthermore,indirect immunofluorescence assays confirmed their specific binding ability to FCV antigens.In conclusion,this study successfully screened scFv antibodies targeting the VP1-CDE region of FCV using phage display technology.The obtained scFvs exhibited high specificity and provided candidate molecules for the development of FCV diagnostic anti-bodies,thereby laying an important experimental foundation for future research.关键词
猫杯状病毒/VP1-CDE蛋白/单链抗体/筛选/鉴定Key words
feline calicivirus/VP1-CDE protein/single-chain fragment variable/screening/iden-tification分类
农业科技引用本文复制引用
赵珍萍,刘超,靳亚丽,刘怡涵,王红梅,朱新龙,张小丽,魏衍全..猫杯状病毒VP1-CDE蛋白单链抗体的筛选与鉴定[J].中国兽医科学,2026,56(8):1058-1066,9.基金项目
甘肃省技术创新引导计划-东西协作专题项目(23CXNA0016) （23CXNA0016）
甘肃省联合科研基金重大项目(25JRRA1086) （25JRRA1086）
兰州市青年科技人才创新项目(2024-QN-165) （2024-QN-165）
2026年甘肃省高校研究生"创新之星"项目(2026CXZX-848) （2026CXZX-848）
