中国兽医科学2026，Vol.56Issue(8)：1127-1135,9.DOI:10.16656/j.issn.1673-4696.2026.0116
妊娠母猪流产胎儿组织中PCV3的分子鉴定与病毒载量分析
Molecular identification and viral load analysis of porcine circovirus type 3 in tissues of an aborted fetus from a pregnant sow
摘要
Abstract
To analyze the causes of abortion in pregnant sows,firstly,nucleic acids were extracted from 18 types of tissue samples of the aborted fetus and the target genes of 7 sow reproductive disor-ders pathogens were detected,such as porcine reproductive and respiratory syndrome virus,classical swine fever virus,porcine circovirus types 2,3,and 4(PCV2,PCV3 and PCV4),African swine fever virus,and porcine parvovirus,by RT-PCR,PCR,and multiplex fluorescence quantitative PCR.Secondly,viral load of the major pathogen in tissue samples of the aborted fetus was determined based on the estab-lished standard plasmids and the plotted standard curves.Finally,whole-genome sequencing and analy-sis of the major pathogen were performed.The results revealed that among the 7 pathogens tested,the aborted fetus was primarily infected with PCV3.This pathogen,designated as PCV3-GS0304/2024,was de-tected in 18 types of tissue samples from the aborted fetus,with its predominant presence observed in the lungs,stomach,throat,tonsils,oral mucosa,and mesenteric lymph nodes,ranging from approximately 1 × 105 to 4× 105 copies/μL.The PCV3-GS0304/2024 belonged to the PCV3b subtype.Both its genome and Cap amino acid sequences exhibited high conservation,notably featuring the H19R mutation.This study clarified the single infection status and tissue distribution characteristics of the PCV3b subtype in porcine abortion cases in Gansu Province,providing a crucial reference for understanding the preva-lence and infection of PCV3 and facilitating its prevention and control.关键词
妊娠母猪繁殖障碍/猪圆环病毒3型/PCV3b亚型/分子诊断Key words
reproductive disorders in pregnant sows/porcine circovirus type 3/PCV3b subtype/molecular diagnosis分类
农业科技引用本文复制引用
蔡梦婷,包慧芳,付元芳,李平花,李冬,白兴文,卢曾军,袁红,孙普,张嘉洋,王涛,郭海洋,赵泽琛,翁涵,李坤..妊娠母猪流产胎儿组织中PCV3的分子鉴定与病毒载量分析[J].中国兽医科学,2026,56(8):1127-1135,9.基金项目
兰州市城关区科技支撑计划项目(2025-zc-28) （2025-zc-28）
甘肃省科技计划项目(23JRRA1515) （23JRRA1515）
甘肃省重大科技专项(21ZD3NA001,22ZD6NA001,22ZD6NA012) （21ZD3NA001,22ZD6NA001,22ZD6NA012）
