中国蔬菜Issue(8)：193-199,7.DOI:10.19928/j.cnki.1000-6346.2026.2017
PMA-PCR检测方法的优化及瓜类种子细菌性果斑病的检测应用
Optimization of PMA-PCR Detection Method and Its Application in Detecting Bacterial Fruit Blotch in Cucurbit Seeds
摘要
Abstract
Bacterial fruit blotch of watermelon is an important seed-borne bacterial disease that severely affects cucurbit crops.A PMA-PCR method was developed for the specific detection of live cells of the bacterial fruit blotch pathogen of watermelon by combining propidium monoazide(PMA)with PCR technology.The results showed that when the PMA concentration was 10 μmol·L-1,followed by incubation in the dark for 10 min and exposure for 20 min,the amplification of DNA from dead bacteria could be effectively inhibited without affecting the detection of live bacteria.The minimum detection limit for live bacteria was 1×104 CFU·mL-1.The established PMA-PCR method was applied to detect watermelon,bottle gourd,and melon seeds,and the results demonstrated that it could quickly and stably distinguish between contaminated and sterilized seeds,accurately identifying the presence of live bacteria.This detection method provides reliable technical support for seed health testing and early control of bacterial fruit blotch of watermelon.关键词
西瓜/细菌性果斑病/叠氮溴化丙锭/活菌检测Key words
watermelon/bacterial fruit blotch/propidium monoazide(PMA)/viable bacteria detection引用本文复制引用
乔琳芝,汤冰倩,周倩,何长征,唐宁,戴思慧,卜凤姣..PMA-PCR检测方法的优化及瓜类种子细菌性果斑病的检测应用[J].中国蔬菜,2026,(8):193-199,7.基金项目
岳麓山实验室种业专项(YLS-2025-ZY02015),国家西甜瓜产业技术体系项目(CARS-25),岳麓山实验室人才专项(2025RC2058,2024RC2011) （YLS-2025-ZY02015）
