中国农业大学学报2026，Vol.31Issue(10)：318-329,12.DOI:10.11841/j.issn.1007-4333.2026.10.22
牛骨骼肌细胞-肌内脂肪细胞互作中关键circRNAs筛选及ceRNA网络解析
Screening of key circRNAs and analysis of the ceRNA network in the interaction between bovine skeletal muscle cells and intramuscular adipocytes
摘要
Abstract
To investigate the effect of bovine skeletal muscle cells on the circular RNA(circRNA)expression profile in intramuscular adipocytes under co-culture conditions,and to elucidate the potential regulatory mechanisms of circRNA in intramuscular fat deposition,this study established a Transwell co-culture model of bovine skeletal muscle cells and intramuscular adipocytes.Meanwhile,circRNA sequencing was performed on intramuscular adipocytes cultured separately as the control group and those co-cultured with skeletal muscle cells as the experimental group.Differential expression analysis was conducted using edgeR software,with a threshold of llog2FCI≥1 andP＜0.05 to identify differentially expressed circRNAs.GO and KEGG enrichment analyses were performed for the host genes of these circRNAs.A circRNA-miRNA-mRNA ceRNA regulatory network was constructed using the OmicShare online platform to further screen key regulatory factors.The results showed that:1)A total of 10 081 circRNAs were identified,including 3 881 unique to the control group,3 465 unique to the co-culture group,and 2 735 shared by both groups.2)A total of 51 differentially expressed circRNAs were identified,including 16 upregulated and 35 downregulated in the co-culture group compared to the control group.3)The differentially expressed circRNAs derived genes were mainly enriched in GO terms such as histone methyltransferase activity and signaling pathways such as ECM-receptor interaction.4)A total of 42 potential ceRNA regulatory pairs were identified,with novel_circ_002953 occupying a central position in the network.5)Novel_circ_002953 may affect the expression of adipogenic differentiation-related genes such as PSAP and DHCR24 by sponging novel-m0002-3p.Novel_circ_002953 and novel_circ_006185 may regulate the expression of lipid metabolism key genes,including WNT5A and ZBTB16,by sponging novel-m0108-3p.In summary,the co-culture environment can alter the circRNA expression profile of bovine intramuscular adipocytes.This study provides novel candidate circRNA molecules for dissecting the skeletal muscle-adipose interaction regulatory network.关键词
牛/肌内脂肪细胞/骨骼肌细胞/共培养/circRNA/ceRNA网络Key words
bovine/intramuscular adipocytes/skeletal muscle cells/co-culture/circRNA/ceRNA network分类
农业科技引用本文复制引用
宋雅萍,魏大为,姜超,焦若普,杨东梅,龚红芳,马艺伦,郑鑫焱,高文杰,王哲彬..牛骨骼肌细胞-肌内脂肪细胞互作中关键circRNAs筛选及ceRNA网络解析[J].中国农业大学学报,2026,31(10):318-329,12.基金项目
宁夏自然科学基金项目(2025AAC040003,2025AAC030558) （2025AAC040003,2025AAC030558）
国家自然科学基金项目(32460819) （32460819）
中央引导地方专项(2024FRD05052) （2024FRD05052）
宁夏重点研发计划(2023BCF01006,2024BBF01007) （2023BCF01006,2024BBF01007）
宁夏青年拔尖人才项目(宁人社函[2026]33) （宁人社函[2026]33）
