中国动物传染病学报2026，Vol.34Issue(3)：44-50,7.
基于双抗体夹心ELISA检测PCV2疫苗的体外相对效力
Detection of the Relative Potency of PCV2 Vaccine Based on Double Antibody Sandwich ELISA
摘要
Abstract
PCV2 has caused huge economic losses to the pig industry since its discovery.The promotion and use of PCV2 vaccines have effectively reduced the harm of PCV2 to the pig industry.At present,there are many kinds of commercial PCV2 vaccines on the market,which show varying the immune effects.To evaluate and screen PCV2 vaccines more economically and quickly,a double antibody sandwich ELISA for detecting PCV2 cap protein was established in this study.The antigen content of cap protein in 16 common commercial PCV2 vaccines on the market was tested to evaluate the relative efficacy of the vaccine.The results showed that this method was suitable for testing the relative potency test of inactivated PCV2 whole virus vaccine,cap protein subunit vaccine and PCV2 virus-like particles(VLPs)vaccine.The relative potency of the subunit vaccine was significantly higher than that of the whole virus-inactivated vaccine.The relative potency test method reduced the cost of PCV2 vaccine testing and improves the efficiency.In summary,this method was a supplementary or even alternative method for the in vivo potency test,which may be used to determine for the of PCV2 vaccine potency and provide reference and guidance for the selection of a PCV2 vaccine on pig farms.关键词
PCV2/疫苗/体外相对效力Key words
PCV2/vaccine/relative efficacy in vitro分类
农业科技引用本文复制引用
宁李纳,刘甜甜,凌同,李欣,陈志雄,范杰,王洪亮,葛猛..基于双抗体夹心ELISA检测PCV2疫苗的体外相对效力[J].中国动物传染病学报,2026,34(3):44-50,7.基金项目
国家自然科学基金面上项目(32072871) （32072871）
湖南省重点研发计划项目(2023NK2017) （2023NK2017）
云南省重大科技专项计划(202202AE090032) （202202AE090032）
