中国动物传染病学报2026，Vol.34Issue(3)：90-95,6.
鉴别猪繁殖与呼吸综合征病毒不同毒株的RT-RAA-LF检测方法
RT-RAA-LF Assay for Differentiation of Classical and Highly Pathogenic Strains of Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
In order to develop a rapid and easy RT-RAA-LF method for the detection of classic and highly pathogenic strains of porcine reproductive and respiratory syndrome virus(PRRSV),primers and probes were designed based on the PRRSV NSP2 gene.The method developed was then optimized for its sensitivity,specificity and also evaluated for its coincidence with the conventional PCR.The results showed that the sensitivity was 1.71×103 copies/μL for pMD18T-NSP2-TJ and 2.19×104 copies/μL for pMD18T-NSP2-VR2332,and its coincidence was 100％with the conventional PCR.The specificity of the dipstick method was 97.7％for highly virogenic strains and 98.4％for classical strains.Therefore,this test could be used to distinguish these strains of PRRSV and the results could be visualized for reading.关键词
猪繁殖与呼吸综合征病毒/Nsp2基因/RT-RAA-LF/经典株/高致病毒株/敏感性/特异性Key words
Porcine reproductive and respiratory syndrome virus/NSP2 gene/RT-RAA-LF/classic strains/highly virulent strains/sensitivity/specificity分类
农业科技引用本文复制引用
于娜,刘光亮,曹宗喜,张紫薇,黄春媛,马佳镁,范悦轩,郑佳馨,李凌伟,张艳,齐萌..鉴别猪繁殖与呼吸综合征病毒不同毒株的RT-RAA-LF检测方法[J].中国动物传染病学报,2026,34(3):90-95,6.基金项目
国家自然科学基金项目(32060796) （32060796）
海南省重点研发项目(ZDYF2023XDNY038) （ZDYF2023XDNY038）
海南省重大科技计划项目(ZDKJ2021030) （ZDKJ2021030）
国家重点研发计划(2021YFA0805905) （2021YFA0805905）
