中国动物传染病学报2026，Vol.34Issue(3)：106-114,9.
非洲猪瘟病毒抗体化学发光检测法的建立
Development of a Chemiluminescence Method for Detection of African Swine Fever Virus Antibodies
摘要
Abstract
African swine fever(ASF)is a highly pathogenic infectious disease frequent outbreaks have occurred in China since 2018,causing serious economic losses to pig industry.In the present study,a rapid and sensitive chemiluminescent method was developed for detection of antibodies against African swine fever virus(ASFV).In this method,ASFV P30 protein was used as the coating antigen,sheep anti-porcine IgG-HRP antibody was used as the enzyme labeled antibody,and luminol was used as the substrate solution.The results showed that the method could detect the serum antibodies specific to ASFV within 30 min at room temperature,and the sensitivity was higher than that of the commercial ASFV antibody detection kit.In addition,there was no cross-reactivity with the positive serum samples of Porcine reproductive and respiratory syndrome virus,Porcine circovirus,Porcine parvovirus,Swine fever virus and Pseudorabies virus.The coefficients of variation within and between batches were less than 10％,indicating its good repeatability.Finally,108 clinical serum samples were tested using this method and a commercial ASFV antibody test kit and their coincidence rate was 93.5％.The ASFV chemiluminescence antibody detection method developed in this study had strong specificity,high sensitivity,good reproducibility,simplicity and rapidity,thus might be applied to the detection of serum ASFV antibodies in clinical practice.关键词
非洲猪瘟病毒/真核表达/化学发光/抗体检测方法Key words
African swine fever virus/eukaryotic expression/chemiluminescence/antibody detection分类
农业科技引用本文复制引用
张宝真,陈卓凡,田欣,何嘉敏,杨佳臻,庞旋飞,王贵平,李中圣,白挨泉..非洲猪瘟病毒抗体化学发光检测法的建立[J].中国动物传染病学报,2026,34(3):106-114,9.基金项目
以农产品为单元的广东省现代农业产业技术体系创新团队建设资助项目(生猪)(BKS209152) （生猪）
