中国动物传染病学报2026，Vol.34Issue(3)：133-141,9.
一株传染性支气管炎病毒的分离鉴定及致病性研究
Isolation,Identification and Pathogenicity of an Infectious Bronchitis Virus
摘要
Abstract
Infectious bronchitis virus(IBV)is a highly infectious pathogen that causes severe respiratory illness,nephritis syndrome and decreased egg production in poultry.In this study,the QX-type IBV strain JSYC2305 was isolated and identified from infected chickens on a chicken farm in Yancheng City,Jiangsu province.The genetic variation and epidemiological characteristics of the virus were studied by means of RT-PCR,isolation,purification,identification and EID50 assay.The results showed that JSYC2305 had typical IBV infection characteristics,including replication ability and pathogenicity on chicken embryos.The amplification and genetic evolution analysis of S1 gene showed that JSYC2305 strain belonged to QX type IBV,which was consistent with the main prevalent genotypes in China.In addition,the study evaluated the pathogenicity of JSYC2305 strain to 10-day-old chicken.The results showed that this strain caused significant respiratory signs and high morbidity in infected SPF chickens.The viremia was detected in multiple tissues of the infected chickens,especially in lungs and the bursas.These results revealed the pathogenicity and tissue tropism of JSYC2305 strain and provided new insights into the pathogenesis of IBV.In additionally,it was worth noting that JSYC2305 strain had low similarity to the commonly used vaccines in China,suggesting that the protection of the existing vaccines against the new variant strains might be insufficient.This study provided a theoretical basis for the prevention and treatment of IBV and the research and development of new vaccines and had important significance for the disease prevention and control of poultry industry.关键词
传染性支气管炎病毒/分离鉴定/S1基因/致病性Key words
Infectious bronchitis virus/isolation and identification/S1 gene/pathogenicity分类
农业科技引用本文复制引用
王晨阳,刘芹防,王彩云,牛世奇,潘晟辉,吕雪华,张云翔,武子华,苑纯秀,滕巧泱,李泽君..一株传染性支气管炎病毒的分离鉴定及致病性研究[J].中国动物传染病学报,2026,34(3):133-141,9.基金项目
"十四五"国家重点研发计划(2023YFD1800605) （2023YFD1800605）
内蒙古自治区自然科学基金(2024LHMS03002) （2024LHMS03002）
