中国兽医科学2026，Vol.56Issue(8)：995-1003,9.DOI:10.16656/j.issn.1673-4696.2026.0129
鸭LMO4的克隆及其在肠炎沙门菌感染输卵管上皮细胞中对NF-κB通路的调控作用
Cloning of duck LMO4 and its regulatory effect on NF-κB signaling pathway in oviduct epithelial cells infected with Salmonella Enteritidis
摘要
Abstract
This study aimed to explore the role of the duck LMO4(duck LIM domain only 4,duLMO4)in resistance to SalmonellaEnteritidis(SE)infection.The duLMO4 gene was cloned by PCR and a phyloge-netic tree was constructed.The expression of duLMO4 in different duck tissues was detected by RT-qPCR.Using the duck oviduct epithelial cells(DOECs)model,combined with gene overexpression,interference and ΔSspH2/C-ΔSspH2 SE infection assays,the expression levels of inflammatory factors such as NF-κB1,IL-6 and IL-1β were detected.Phylogenetic tree showed that it had the highest homology with the wild goose(99.39％).The duLMO4 gene was expressed in all seven tissues:heart,liver,spleen,lung,kid-ney,duodenum and oviduct epithelium,with the highest mRNA expression level observed in the oviduct epithelium.Overexpression of duLMO4 gene significantly upregulated TNF-α,NF-κB1,IL-6 and IL-1β mRNA expression(increased by 43.8％,78.2％,179.9％and 108.6％,P＜0.05)and NF-κB1 and IL-1β protein levels(increased by 47.2％and 79.7％,P＜0.05),while interference of duLMO4 gene significantly inhib-ited TNF-α,NF-κB1,IL-6 and IL-1β mRNA expression(decreased by 78.8％,66.0％,83.7％,and 91.4％,P＜0.05)and NF-κB1 and IL-1β protein levels(decreased by 64.8％and 77.9％,P＜0.05).After SE infection,compared with the wild type,the expression of LMO4 was significantly reduced by SspH2 deletion(de-creased by 92％,P＜0.05),while LMO4 expression was significantly elevated in the complementation strain C-ΔSspH2(increased by 1.29-fold,P＜0.01).In summary,the interference with duLMO4 likely reg-ulates inflammatory response by inhibiting the expression of inflammatory factor IL-1β downstream of the NF-κB pathway,and SspH2 acts as an important effector molecule of SE to modulate host inflammatory responses.关键词
LMO4/肠炎沙门菌/NF-κB通路/炎症因子Key words
LMO4/Salmonella Enteritidis/NF-κB pathway/inflammatory factor分类
农业科技引用本文复制引用
姜又鸾,刘萍萍,罗舒文,夏丽佳,曹正锋,徐琪,张钰..鸭LMO4的克隆及其在肠炎沙门菌感染输卵管上皮细胞中对NF-κB通路的调控作用[J].中国兽医科学,2026,56(8):995-1003,9.基金项目
国家自然科学基金项目(32372863) （32372863）
扬州市科技计划项目(YZ2023263) （YZ2023263）
