中国兽医科学2026，Vol.56Issue(8)：1004-1013,10.DOI:10.16656/j.issn.1673-4696.2026.0155
钙依赖性蛋白激酶4在柔嫩艾美耳球虫感染鸡盲肠组织中水平变化及其对细胞凋亡的影响
Changes in the level of calcium-dependent protein kinase 4 in chicken cecal tissue infected with Eimeria tenella and its effect on cell apoptosis
摘要
Abstract
This study aimed to investigate the expression profile of Eimeria tenella calcium-dependent protein kinase 4(CDPK4)across its developmental cycle in chickens,as well as its regulatory role in apoptosis in chicken fibroblast DF-1 cells.Recombinant CDPK4 protein was prokaryotically expressed and purified using an Escherichia coli system,and murine polyclonal antibodies against CDPK4 were prepared.Immuno-fluorescence staining was employed to examine the temporal expression of CDPK4 in cecal tissues from chickens infected with E.tenella at different time points.Meanwhile,the recombinant plasmid pEGFP-N1-CDPK4 was constructed and transfected into DF-1 cells.The regulatory effects of CDPK4 on the expression of key apoptotic mediators and Caspase-3 enzymatic activity in DF-1 cells were evaluated using RT-qPCR,We stern blot,and colorimetric Caspase-3 activity assays.The results showed that CDPK4 expression in cecal tissues of E.tenella-infected chickens increased gradually with parasite develop-ment and proliferation.In DF-1 cells expressing CDPK4,the mRNA and protein expression levels of the anti-apoptotic factor Bcl-2 were upregulated significantly(P＜0.01),whereas the mRNA and protein expression levels of the pro-apoptotic factor Bax were downregulated significantly(P＜0.01),leading to an elevated Bcl-2/Bax ratio.Caspase-3 enzymatic activity was reduced significantly(P＜0.05),indicating that CDPK4 suppresses apoptosis in DF-1 cells.关键词
柔嫩艾美耳球虫/钙依赖性蛋白激酶4/细胞凋亡Key words
Eimeria tenella/calcium-dependent protein kinase 4/apoptosis分类
农业科技引用本文复制引用
王雨婷,赵雁萍,关文超,杨裕,崔小珍,秦冉,徐梦博,王吉霞,王璐瑶,赵睿,张黎,白瑞,吕晓玲..钙依赖性蛋白激酶4在柔嫩艾美耳球虫感染鸡盲肠组织中水平变化及其对细胞凋亡的影响[J].中国兽医科学,2026,56(8):1004-1013,10.基金项目
动物重要疫病研究与防控山西省重点实验室平台项目(202504010931010) （202504010931010）
山西省基础研究计划青年项目(202303021222078) （202303021222078）
山西农业大学横向科研项目(2025HX105) （2025HX105）
山西农业大学科技创新提升工程项目(CXGC2025013,CXGC2025012) （CXGC2025013,CXGC2025012）
