浙江农业学报2026，Vol.38Issue(7)：1366-1377,12.DOI:10.3969/j.issn.1004-1524.20250541
辣椒根际合成菌群的构建及其促生效果评价
Construction of a synthetic community from the pepper rhizosphere and evaluation of its growth-promoting effects
摘要
Abstract
Excessive fertilization and soil-borne diseases have long restricted the production of pepper(Capsicum an-nuum L.).Traditional chemical amendments,though commonly used to alleviate these constraints,tend to cause secondary problems such as soil microecological imbalance and degradation of physicochemical properties after long-term application.In light of this,the present study constructed a functional synthetic microbial community from the pepper rhizosphere and evaluated its growth-promoting effects,aiming to provide a theoretical basis for its application in sustainable pepper production.Building upon 12 strains of plant growth-promoting rhizobacteria(PGPR)previ-ously isolated and identified from the pepper rhizosphere in laboratory studies,strains exhibiting nitrogen-fixing,phosphorus-solubilizing,potassium-releasing,and indole-3-acetic acid(IAA)-producing properties were selected,and four synthetic microbial communities(Syncom 1-4)were constructed based on principles of functional comple-mentarity.Their growth-promoting effects on pepper were systematically evaluated through pot experiments.The re-sults indicated that Syncom 4 exhibited optimal growth-promoting effects.Compared with single-strain treatments,Syncom 4 demonstrated a significant(p<0.01 or p<0.001)enhanced capacity for biofilm formation,with no ob-served inter-strain antagonism.This consortium significantly promoted root hair development in pepper seedlings and increased plant biomass.Furthermore,Syncom 4 significantly reduced the pH level and available potassium content inrhizosphere soil.16S rRNA amplicon sequencing revealed that Syncom 4 treatment altered bacterial community structures within pepper roots and the rhizosphere,enriched certain beneficial bacterial taxa,such as Pseudomonas,Enterobacter,Bacillus,Flavobacterium,and Microbacterium.In summary,Syncom 4 exhibits excellent growth-pro-moting effects on pepper seedlings,providing microbial strain resources and technical support for the development of composite microbial fertilisers for peppers.关键词
辣椒/根际促生菌/合成菌群/促生效果Key words
Capsicum annuum L./plant growth promoting rhizobacteria/synthetic bacterial community/growth-promoting effect分类
农业科技引用本文复制引用
姜贵武,张琳,徐伟慧,王志刚..辣椒根际合成菌群的构建及其促生效果评价[J].浙江农业学报,2026,38(7):1366-1377,12.基金项目
黑龙江省重点研发计划(GA23B018) （GA23B018）
中国科学院科研项目 （）
