浙江农业学报2026，Vol.38Issue(7)：1400-1409,10.DOI:10.3969/j.issn.1004-1524.20250429
绣球湖蓝炭疽病病原菌的分离、鉴定与防治
Isolation,identification and control of the pathogen of anthracnose in Hydrangea macrophylla Hulan
摘要
Abstract
To elucidate the causal agent of anthracnose in Hydrangea macrophylla and explore effective control strate-gies,this study isolated pathogens from anthracnose-infected sepals of the cultivar Hulan using tissue isolation and confirmed their pathogenicity via Koch's postulates.The pathogen was further identified based on morphological char-acteristics and phylogenetic analysis constructed from multi-gene sequences of ITS,Actin,TUB2,CHS-1 and GAP-DH.The biological characteristics of the pathogen were investigated using the mycelial growth rate method,assessing the effects of different culture media,carbon and nitrogen sources,temperature,light and pH value on mycelial growth.In addition,five commonly used fungicides and 32 Hydrangea macrophylla cultivars were tested to evaluate in vitro fungicidal efficacy and host resistance.The results indicated that the anthracnose pathogen was Colletotrichum karstii.Optimal mycelial growth occurred at 25℃and pH value of 7 on potato dextrose agar(PDA)supplemented with xylose as the carbon source and beef extract as the nitrogen source,while the pathogen exhibited no sensitivity to light.The lethal temperature was determined to be 56℃for 10 min.Among the tested fungicides,50%thiram+procymidone demonstrated the highest inhibitory effect,with the median effective concentration(EC50)of 0.075 4 mg·L-1.Resistance evaluation revealed that the cultivars Lansehaiyang,Wanziqianhong,Bodalan,Qianying,Xue-hua,Renmiantaohua,Zijuan and Xingkong were immune.This study was the first report of C.karstii causing an-thracnose on Hydrangea macrophylla in China.关键词
大叶绣球/炭疽病/喀斯特炭疽菌/生物学特性/药剂筛选/抗病性Key words
Hydrangea macrophylla/anthracnose/Colletotrichum karstii/biological characteristic/fungicide screening/disease resistance分类
农业科技引用本文复制引用
刘光炎,熊海燕,周梅,李正敏,王金燕,陈晶晶,张玉,王超..绣球湖蓝炭疽病病原菌的分离、鉴定与防治[J].浙江农业学报,2026,38(7):1400-1409,10.基金项目
云南省重大科技专项(202202AE090028) （202202AE090028）
云南省教育厅自然科学项目(2025Y0866) （2025Y0866）
云南省大学生创新训练计划项目(20200554052) （20200554052）
